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中华肺部疾病杂志(电子版) ›› 2024, Vol. 17 ›› Issue (05) : 719 -724. doi: 10.3877/cma.j.issn.1674-6902.2024.05.009

论著

非小细胞肺癌铂类药物耐药mi RNA表达特征及预测分析
杨慧1, 郭丽娟1, 冯晓丹1, 李静1, 黄成谋2, 蔡兴锐2, 覃英娇3, 王远礼3   
  1. 1.570100 海口,海南医学院第一附属医院放疗科
    2.570100 海口,海南医学院第一附属医院肿瘤内科
    3.570100 海口,海南医学院第一附属医院呼吸内科
  • 收稿日期:2024-02-13 出版日期:2024-10-25

Expression characteristics and prediction analysis of platinum-resistant mi RNA in non-small cell lung cancer

Hui Yang1, Lijuan Guo1, Xiaodan Feng1, Jing LI1, Chengmo Huang2, Xingrui Cai2, Yingjiao Qin3, Yuanli Wang3   

  1. 1.Department of Radiotherapy,The First Affiliated Hospital of Hainan Medical College,Haikou 570100, China
    2.Department of Oncology, The First Affiliated Hospital of Hainan Medical College,Haikou 570100, China
    3.Department of Respiratory Medicine, The First Affiliated Hospital of Hainan Medical College, Haikou 570100, China
  • Received:2024-02-13 Published:2024-10-25
引用本文:

杨慧, 郭丽娟, 冯晓丹, 李静, 黄成谋, 蔡兴锐, 覃英娇, 王远礼. 非小细胞肺癌铂类药物耐药mi RNA表达特征及预测分析[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(05): 719-724.

Hui Yang, Lijuan Guo, Xiaodan Feng, Jing LI, Chengmo Huang, Xingrui Cai, Yingjiao Qin, Yuanli Wang. Expression characteristics and prediction analysis of platinum-resistant mi RNA in non-small cell lung cancer[J/OL]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(05): 719-724.

目的

分析非小细胞肺癌(non-small cell lung cancer, NSCLC)微小RNA(micro RNA,miRNA)表达特征,预测铂类药物耐药性。

方法

选择2021 年9 月至2023 年4 月我院收治的105 例NSCLC 患者为对象,铂类药物治疗敏感66 例为对照组,耐药39 例为观察组。 采用聚合酶链式反应(polymerase chain reaction, PCR)检测miRNA 表达水平。 采用多因素Logistic 回归分析NSCLC 铂类药物耐药的影响因素,通过受试者工作特征曲线(receiver operating characteristic curve, ROC)分析miRNA 表达特征预测NSCLC 铂类药物耐药性。

结果

观察组miRNA-125b(0.92±0.21)、miRNA-106a(0.87±0.22)、miRNA-181a(0.89±0.13)、miRNA-196a(1.01±0.31)表达水平高于对照组miRNA-125b(1.75±0.35)、miRNA-106a(2.45±0.47)、miRNA-181a(1.04±0.21)、miRNA-196a(2.48±0.55),观察组miRNA-148b(1.78±0.47)低于对照组(0.97±0.16)(P<0.05);多因素Logistic 回归分析显示,miRNA-125b(OR=1.642)、miRNA-106a(OR=2.052)、miRNA-181a(OR=2.092)、miRNA-196a(OR=1.508)及miRNA-148b(OR=0.385)表达水平是NSCLC 铂类药物耐药情况的影响因素(P<0.05);ROC 曲线显示,miRNA-125b、miRNA-106a、miRNA-181a、miRNA-196a 及miRNA-148b 表达水平预测NSCLC 铂类药物耐药的AUC 分别为0.795、0.895、0.733、0.878、0.888,miRNA-106a 有预测价值,最佳诊断界值为1.220 时,预测NSCLC 铂类药物耐药的敏感性76.62%,特异性为96.97%。

结论

miRNA 表达特征可预测NSCLC 铂类药物耐药情况, miRNA-125b、miRNA-106a、miRNA-181a、miRNA-196a 表达水平高及miRNA-148b 表达水平低的NSCLC 患者易发生铂类药物耐药。

Objective

To analyze the expression characteristics of micro RNA (miRNA) in non-small cell lung cancer (NSCLC) and predict the resistance of platinum drugs.

Methods

All of 105 patients with NSCLC admitted to our hospital from September 2021 to April 2023 were selected as subjects, 66 patients with platinum therapy sensitivity were selected as control group, and 39 patients with drug resistance were selected as observation group. miRNA expression was detected by polymerase chain reaction (PCR). Multivariate Logistic regression was used to analyze the influencing factors of platinum drug resistance in NSCLC, and the expression characteristics of miRNA were analyzed by receiver operating characteristic curve (ROC) to predict platinum drug resistance in NSCLC.

Results

The expression levels of miRNA-125b (0.92±0.21), miRNA-106a (0.87±0.22), miRNA-181a (0.89±0.13) and miRNA-196a (1.01±0.31) in observation group were higher than those in control group (1.75±0.35). miRNA-106a (2.45±0.47), miRNA-181a (1.04±0.21),miRNA-196a (2.48±0.55), miRNA-148b (1.78±0.47) in observation group was lower than that in control group (0.97±0.16) (P<0.05). Multivariate Logistic regression analysis showed that the expression levels of miRNA-125b (OR=1.642), miRNA-106a (OR=2.052), miRNA-181a (OR=2.092), miRNA-196a (OR=1.508) and miRNA-148b (OR= 0.385) were platinum drug resistant in NSCLC influencing factors of the situation (P<0.05); ROC curve showed that the expression levels of miRNA-125b, miRNA-106a, miRNA-181a, miRNA-196a and miRNA-148b predicted the AUC of platinum drug resistance in NSCLC were 0.795,0.895, 0.733, 0.878 and 0.888,respectively. The predictive value of miRNA-106a was high. When the optimal diagnostic threshold was 1.220, the sensitivity and specificity of mirNA-106A for predicting platinum drug resistance in NSCLC were 76.62% and 96.97%.

Conclusion

The expression characteristics of miRNA can predict platinum drug resistance in NSCLC. NSCLC patients with high expression levels of mirNA-125b,mirNA-106A, mirNA-181A, mirNA-196a and low expression levels of mirNA-148b are prone to platinum drug resistance.

表1 两组NSCLC 患者一般资料结果比较[n(%)]
表2 两组NSCLC 患者miRNA 表达水平结果比较(±s
表3 影响NSCLC 患者铂类药物耐药的Logistic 多因素分析
表4 miRNA 表达特征对NSCLC 铂类药物耐药的预测
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