研究荧光定量TB-PCR法检测超声支气管镜引导下经支气管透壁针吸活检(EBUS-TBNA)标本的临床应用价值。

对2017年01月至2018年06月在我院呼吸内镜中心接受EBUS-TBNA检测的65例患者的临床资料进行回顾性分析，比较病理检查和病理检查联合荧光定量TB-PCR检测法对临床诊断纵膈淋巴结结核的敏感性、特异性和准确性。

病理检查对纵膈淋巴结核的敏感性、特异性、准确性分别是75%、83.3%、78.9%，而荧光定量TB-PCR法联合病理学检查对纵膈淋巴结核诊断的敏感性、特异性、准确率分别为90.5%、100%、94.4%。

针对EBUS-TBNA标本，采用病理学检查联合荧光定量TB-PCR检测法能够明显提高诊断淋巴结结核的敏感性、特异性、准确性，有利于结节病和纵隔结核、非结核分枝杆菌病的鉴别，具有很高的临床应用价值。

To evaluate the clinical value of fluorescence quantitative tuberculosis-polymerase chain reaction (TB-PCR) for mediastinal lymphadenopathy in endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) specimens.

The clinical data of 65 patients who received EBUS-TBNA in the Respiratory Endoscopy Center of Tangdu Hospital affiliated to Air Force Military Medical University from January 2017 to June 2018 were retrospectively analyzed. The sensitivity, specificity and diagnostic accuracy of the pathological examination of biopsy specimens for lymph node tuberculosis with or without fluorescence quantitative TB-PCR were statistically analyzed.

The sensitivity, specificity, and accuracy of EBUS-TBNA specimens by the pathological examination for the diagnosis of mediastinal lymphatic tuberculosis were 75%, 83.3%, and 78.9%, respectively, while they were 90.5%, 100%, and 94.4%, respectively, by the fluorescence quantitative TB-PCR combined with pathological examination.

The pathologic diagnosis of EBUS-TBNA specimens combined with fluorescence quantitative TB-PCR is significantly improved in the sensitivity, specificity and accuracy for the diagnosis of lymph node tuberculosis, which is helpful in the differentiation of sarcoidosis and mediastinal tuberculosis and non-tuberculous mycosis, and therefore, it is worthy of clinical application.