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中华肺部疾病杂志(电子版) ›› 2024, Vol. 17 ›› Issue (04) : 600 -604. doi: 10.3877/cma.j.issn.1674-6902.2024.04.018

临床研究

肺腺癌miR-3653表达与高危型人乳头瘤病毒感染及预后的关系
刘静1, 徐爽1, 缪亚军1,()   
  1. 1. 226000 南通,南通大学第二附属医院肿瘤内科
  • 收稿日期:2024-05-17 出版日期:2024-08-25
  • 通信作者: 缪亚军
  • 基金资助:
    江苏省卫生计生委医学科研课题(H201782); 南通市卫生健康委员会科研课题(MS2022033)

Relationship between Mir­3653 expression and high­risk human papillomavirus infection and prognosis in lung adenocarcinoma

Jing Liu, Shuang Xu, Yajun Miao()   

  • Received:2024-05-17 Published:2024-08-25
  • Corresponding author: Yajun Miao
引用本文:

刘静, 徐爽, 缪亚军. 肺腺癌miR-3653表达与高危型人乳头瘤病毒感染及预后的关系[J]. 中华肺部疾病杂志(电子版), 2024, 17(04): 600-604.

Jing Liu, Shuang Xu, Yajun Miao. Relationship between Mir­3653 expression and high­risk human papillomavirus infection and prognosis in lung adenocarcinoma[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(04): 600-604.

目的

分析组织微小RNA(miR)-3653与肺腺癌(lung adenocarcinoma, LUAD)中高危型人乳头瘤病毒感染(high-risk human papillomavirus, HR-HPV)的关系,以及miR-3653对LUAD诊断和预后的临床意义。

方法

选择2020年4月至2021年3月我院收治的102例LUAD患者,收集肿瘤组织和癌旁非癌组织。采用实时荧光定量PCR检测miR-3653。采用流式荧光液相芯片技术测定组织HR-HPV分型。通过受试者工作特征曲线(receiver operating characteristic, ROC)分析miR-3653判断HPV阳性(HPV)和HPV阴性(HPV)LUAD及LUAD癌旁组织。采用Kaplan-Meier曲线分析miR-3653与LUAD无病生存期(disease-free survival, DFS)和总生存期(overall survival, OS)的相关性。

结果

LUAD组织中miR-3653表达1.33(0.76,2.63)较癌旁组织3.39(1.71,7.09)降低(Z=-6.617,P<0.001); LUAD组织miR-3653低表达(<1.33)者淋巴结转移数量多(P<0.05)。LUAD中HR-HPV感染29例(28.43%)高于癌旁组织的8例(7.84%)(χ2=14.560,P<0.001)。LUAD与HR-HPV[0.94(0.69,2.19)]、LUAD与HR-HPV[2.20(1.49,3.68)]、癌旁组织HR-HPV[2.96(1.67,6.63)]、癌旁组织HR-HPV[8.95(8.16,11.52)]中miR-3653逐渐升高(H=60.818,P<0.001)。ROC曲线显示组织miR-3653诊断LUAD的AUC为0.768(0.704~0.832)、HR-HPV感染AUC0.616(0.528~0.703)、诊断LUAD中HR-HPV感染AUC 0.766(0.673~0.859)。中位DFS为12.25个月,病情进展36例(35.29%);中位OS为18.50个月,全因死亡21例(20.59%),HR-HPV感染且miR-3653高表达者中DFS和OS较HR-HPV感染、miR-3653高表达者长。

结论

miR-3653与LUAD发生、预后和HR-HPV感染有关,可诊断HR-HPV感染阳性LUAD,对预后风险分层有意义。

表1 LUAD组织miR-3653表达与临床特征的关系[M50(P25,P75),n(%)]
图1 HR-HPV感染与miR-3653的关系
表2 组织miR-3653诊断LUAD和HR-HPV感染的ROC曲线
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