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中华肺部疾病杂志(电子版) ›› 2024, Vol. 17 ›› Issue (04) : 600 -604. doi: 10.3877/cma.j.issn.1674-6902.2024.04.018

临床研究

肺腺癌miR-3653表达与高危型人乳头瘤病毒感染及预后的关系
刘静1, 徐爽1, 缪亚军1,()   
  1. 1. 226000 南通,南通大学第二附属医院肿瘤内科
  • 收稿日期:2024-05-17 出版日期:2024-08-25
  • 通信作者: 缪亚军
  • 基金资助:
    江苏省卫生计生委医学科研课题(H201782); 南通市卫生健康委员会科研课题(MS2022033)

Relationship between Mir­3653 expression and high­risk human papillomavirus infection and prognosis in lung adenocarcinoma

Jing Liu, Shuang Xu, Yajun Miao()   

  • Received:2024-05-17 Published:2024-08-25
  • Corresponding author: Yajun Miao
引用本文:

刘静, 徐爽, 缪亚军. 肺腺癌miR-3653表达与高危型人乳头瘤病毒感染及预后的关系[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(04): 600-604.

Jing Liu, Shuang Xu, Yajun Miao. Relationship between Mir­3653 expression and high­risk human papillomavirus infection and prognosis in lung adenocarcinoma[J/OL]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(04): 600-604.

目的

分析组织微小RNA(miR)-3653与肺腺癌(lung adenocarcinoma, LUAD)中高危型人乳头瘤病毒感染(high-risk human papillomavirus, HR-HPV)的关系,以及miR-3653对LUAD诊断和预后的临床意义。

方法

选择2020年4月至2021年3月我院收治的102例LUAD患者,收集肿瘤组织和癌旁非癌组织。采用实时荧光定量PCR检测miR-3653。采用流式荧光液相芯片技术测定组织HR-HPV分型。通过受试者工作特征曲线(receiver operating characteristic, ROC)分析miR-3653判断HPV阳性(HPV)和HPV阴性(HPV)LUAD及LUAD癌旁组织。采用Kaplan-Meier曲线分析miR-3653与LUAD无病生存期(disease-free survival, DFS)和总生存期(overall survival, OS)的相关性。

结果

LUAD组织中miR-3653表达1.33(0.76,2.63)较癌旁组织3.39(1.71,7.09)降低(Z=-6.617,P<0.001); LUAD组织miR-3653低表达(<1.33)者淋巴结转移数量多(P<0.05)。LUAD中HR-HPV感染29例(28.43%)高于癌旁组织的8例(7.84%)(χ2=14.560,P<0.001)。LUAD与HR-HPV[0.94(0.69,2.19)]、LUAD与HR-HPV[2.20(1.49,3.68)]、癌旁组织HR-HPV[2.96(1.67,6.63)]、癌旁组织HR-HPV[8.95(8.16,11.52)]中miR-3653逐渐升高(H=60.818,P<0.001)。ROC曲线显示组织miR-3653诊断LUAD的AUC为0.768(0.704~0.832)、HR-HPV感染AUC0.616(0.528~0.703)、诊断LUAD中HR-HPV感染AUC 0.766(0.673~0.859)。中位DFS为12.25个月,病情进展36例(35.29%);中位OS为18.50个月,全因死亡21例(20.59%),HR-HPV感染且miR-3653高表达者中DFS和OS较HR-HPV感染、miR-3653高表达者长。

结论

miR-3653与LUAD发生、预后和HR-HPV感染有关,可诊断HR-HPV感染阳性LUAD,对预后风险分层有意义。

表1 LUAD组织miR-3653表达与临床特征的关系[M50(P25,P75),n(%)]
图1 HR-HPV感染与miR-3653的关系
表2 组织miR-3653诊断LUAD和HR-HPV感染的ROC曲线
1
Howlader N, Forjaz G, Mooradian MJ, et al. The effect of advances in lung-cancer treatment on population mortality[J]. N Engl J Med, 2020, 383(7): 640-649.
2
李 丽,郭孟刚,罗晓斌,等. 基于SEER数据库分析肺腺癌的发病率及生存趋势[J]. 安徽医药2023, 27(9): 1796-1799.
3
Oyouni AAA. Human papillomavirus in cancer: Infection, disease transmission, and progress in vaccines[J]. J Infect Public Health, 2023, 16(4): 626-631.
4
Hill M, Tran N. miRNA interplay: mechanisms and consequences in cancer[J]. Dis Model Mech, 2021, 14(4): dmm047662.
5
Nunvar J, Pagacova L, Vojtechova Z, et al. Lack of conserved miRNA deregulation in HPV-induced squamous cell carcinomas[J]. Biomolecules, 2021, 11(5): 764.
6
Song H, Zhao Z, Ma L, et al. MiR-3653 blocks autophagy to inhibit epithelial-mesenchymal transition in breast cancer cells by targeting the autophagy-regulatory genes ATG12 and AMBRA1[J]. Chin Med J (Engl), 2023, 136(17): 2086-2100.
7
Cui H, Zhang B, Ruan M, et al. Overexpression of miR-3653 is associated with HPV infection and serves as a biomarker in patients with cervical cancer[J]. Int J Womens Health, 2022, 14: 1037-1045.
8
Barta JA, Powell CA, Wisnivesky JP. Global epidemiology of lung cancer[J]. Ann Glob Health, 2019, 85(1): 8-23.
9
Succony L, Rassl DM, Barker AP, et al. Adenocarcinoma spectrum lesions of the lung: Detection, pathology and treatment strategies[J]. Cancer Treat Rev, 2021, 99: 102237.
10
Song B, Qian J, Fu J. Research progress and potential application of microRNA and other non-coding RNAs in forensic medicine[J]. Int J Legal Med, 2024, 138(2): 329-350.
11
Choudhary C, Sharma S, Meghwanshi KK, et al. Long non-coding RNAs in insects[J]. Animals, 2021, 11(4): 1118-1137.
12
Peng F, Fan H, Li S, et al. MicroRNAs in epithelial-mesenchymal transition process of cancer: Potential targets for chemotherapy[J]. Int J Mol Sci, 2021, 22(14): 7526-7544.
13
Ramazi S, Dadzadi M, Sahafnejad Z, et al. Epigenetic regulation in lung cancer[J]. MedComm, 2023, 4(6): e401-e446.
14
Ying X, Ma N, Zhang X, et al. Research progress on the molecular mechanisms of hepatic metastasis in lung cancer: a narrative review[J]. Ann Palliat Med, 2021, 10(4): 4806-4822.
15
郑俊斌. 肺腺癌差异表达的miRNA筛选研究[J]. 广州医科大学学报2021, 49(5): 22-25.
16
Zarrilli G, Galuppini F, Angerilli V, et al. miRNAs involved in esophageal carcinogenesis and miRNA-related therapeutic perspectives in esophageal carcinoma[J]. Int J Mol Sci, 2021, 22(7): 3640.
17
Bai X, Xu Y, Liu Y. MicroRNA-3653-3p inhibited papillary thyroid carcinoma progression by regulating CRIPTO-1[J]. Cell Mol Biol (Noisy-le-grand), 2023, 69(14): 272-276.
18
Song H, Zhao Z, Ma L, et al. MiR-3653 blocks autophagy to inhibit epithelial-mesenchymal transition in breast cancer cells by targeting the autophagy-regulatory genes ATG12 and AMBRA1[J]. Chin Med J (Engl), 2023, 136(17): 2086-2100.
19
Zhu W, Luo X, Fu H, et al. MiR-3653 inhibits the metastasis and epithelial-mesenchymal transition of colon cancer by targeting Zeb2[J]. Pathol Res Pract, 2019, 215(10): 152577.
20
Hu Z, Yang C, Guo S, et al. LINC01615 activates ZEB2 through competitively binding with miR-3653-3p to promote the carcinogenesis of colon cancer cells[J]. Cell Cycle, 2022, 21(3): 228-246.
21
Zhang L, Zhang T, Deng Z, et al. MicroRNA?3653 inhibits the growth and metastasis of hepatocellular carcinoma by inhibiting ITGB1[J]. Oncol Rep, 2019, 41(3): 1669-1677.
22
Delek FSP, Tunçer ŞB, Ödemiş DA, et al. miR-3653-3p expression in PBMCs: Unveiling the diagnostic potential for ovarian cancer[J]. Biochem Genet, 2024, doi: 10.1007/s10528-024-10819-0.
23
刘瑞花,李晓琴,于海泉. miR-3653-3p在人肺癌细胞系中的作用及调节[C]. 2017年第十五届中国北方实验动物科技年会论文集,2017: 427-433.
24
Lin K, Xu T, He BS, et al. MicroRNA expression profiles predict progression and clinical outcome in lung adenocarcinoma[J]. Onco Targets Ther, 2016, 9: 5679-5692.
25
Su X, Liu P, Zhao H, et al. Impact of HR-HPV infection on oncological outcomes in early cervical cancer[J]. Front Oncol, 2023, 13: 1264114.
26
Oyouni AAA. Human papillomavirus in cancer: Infection, disease transmission, and progress in vaccines[J]. J Infect Public Health, 2023, 16(4): 626-631.
27
杨雪娇,吴艳飞. HPV感染及IL-1β、EGFR单核苷酸多态性与非吸烟女性肺癌的相关性研究[J]. 公共卫生与预防医学2022, 33(6): 115-118.
28
Rojas L, Mayorga D, Ruiz-Patiño A, et al. Human papillomavirus infection and lung adenocarcinoma: Special benefit is observed in patients treated with immune checkpoint inhibitors[J]. ESMO Open, 2022, 7(4): 100500.
29
喻茂文,罗朝武,汤洪波,等. 肺癌患者人乳头瘤病毒感染对机体免疫功能的影响及与预后的关系研究[J]. 国际病毒学杂志2022, 29(3): 226-229.
30
Hussen BM, Ahmadi G, Marzban H, et al. The role of HPV gene expression and selected cellular MiRNAs in lung cancer development[J]. Microb Pathog, 2021, 150: 104692.
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