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中华肺部疾病杂志(电子版) ›› 2025, Vol. 18 ›› Issue (03) : 345 -349. doi: 10.3877/cma.j.issn.1674-6902.2025.03.001

论著

早期非小细胞肺癌患者术前术后血清miR-21 和miR-937-3p表达变化的研究
赵雅波1, 王倩2, 闫小龙1, 王元勇3, 何改花4, 郭一泽1,()   
  1. 1. 710038 西安,空军军医大学第二附属医院胸腔外科
    2. 266011 青岛,康复大学青岛医院(青岛市立医院)妇科
    3. 100142 北京,北京大学肿瘤医院胸外一科
    4. 710038 西安,空军军医大学第二附属医院内分泌科
  • 收稿日期:2024-08-16 出版日期:2025-06-25
  • 通信作者: 郭一泽
  • 基金资助:
    国家自然基金资助项目(8217111370)西安市创新能力支撑计划(23YXYJ0171)

Study on serum miR-21 and miR-937-3p expression changes in early stage non-small cell lung cancer patients before and after surgery

Yabo Zhao1, Qian Wang2, Xiaolong Yan1, Yuanyong Wang3, Gaihua He4, Yize Guo1,()   

  1. 1. Department of Thoracic Surgery,The Second Affiliated Hospital of Air Force Military Medical University,Xi'an 710038,China
    2. Department of Gynecology,Qingdao Hospital,University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital),Qingdao 266011,China
    3. Department of Thoracic Surgery,Peking University Cancer Hospital,Beijing 100142,China
    4. Department of Endocrinology,The Second Affiliated Hospital of Air Force Medical University,Xi'an 710038,China
  • Received:2024-08-16 Published:2025-06-25
  • Corresponding author: Yize Guo
引用本文:

赵雅波, 王倩, 闫小龙, 王元勇, 何改花, 郭一泽. 早期非小细胞肺癌患者术前术后血清miR-21 和miR-937-3p表达变化的研究[J/OL]. 中华肺部疾病杂志(电子版), 2025, 18(03): 345-349.

Yabo Zhao, Qian Wang, Xiaolong Yan, Yuanyong Wang, Gaihua He, Yize Guo. Study on serum miR-21 and miR-937-3p expression changes in early stage non-small cell lung cancer patients before and after surgery[J/OL]. Chinese Journal of Lung Diseases(Electronic Edition), 2025, 18(03): 345-349.

目的

微RNA(miRNA)在基因表达的转录后调控中起着关键作用,已成为恶性肿瘤发生、发展和转移的重要调控因子。 收集非小细胞肺癌(non-small cell lung caner,NSCLC)患者术前和术后3 个月的血清样本中mir-21、mir-671-5p、mir-937-3p 和mir-611 的表达谱,分析生物标志物的潜力。

方法

采集62 例早期NSCLC 患者术前和术后3 个月的血液样本。 采用定量实时聚合酶链式反应(polymerase chain reaction,PCR) 技术对血清中mir-21、mir-671-5p、mir-937-3p 和mir-611 的相对表达水平进行量化。

结果

术前和术后3 个月比较,患者术后血清miR-21 下降0.2584 倍(95% CI:0.05664 ~0.4601)、miR-671-5p 下降0.2213 倍(95% CI:0.02188~0.4207)、miR-937-3p 下降0.2182 倍(95% CI:0.05234~0.3840)和miR-611 下降0.6224 倍(95% CI:0.4426~0.8021),分子表达水平低于手术前(P=0.0039,P=0.0154,0.0031,P<0.0001)。 特征曲线(receiver operating characteristic curve,ROC)分析表明,miR-21(AUC=0.6942,P=0.0004) 和miR-937-3p (AUC=0.6522,P=0.0059)能以相对较高的准确度区分NSCLC 病例术前术后水平,基因的血清水平与患者的临床特征之间没有发现明显的相关性。 miR-671-5p 术前的NSCLC 样本中显示出上调,但AUC 值为0.5974(P= 0.0783),显示其不适合作为NSCLC 的生物标志物。miR-611 的AUC 为0.5630(P=0.2548),不适合作为NSCLC 生物标志物。 依数据结果,miR-21 和miR-937-3p 有可能成为筛选NSCLC 的生物标志物。

结论

血清中miR-21 和miR-937-3p 有望在早期NSCLC患者中成为诊断和预后的潜在生物标志物。

Objective

MicroRNAs (miRNAs) play a key role in the post-transcriptional regulation of gene expression and have emerged as important regulators of cancer onset,progression and metastasis. The aim of this study was to evaluate the expression profiles of mir-21,mir-671-5p,mir-937-3p,and mir-611 in preoperative and three-month postoperative serum samples from patients with early-non-small cell lung caner(NSCLC) in order to explore their biomarker potential.

Methods

A total of 62 blood samples from early-NSCLC patients (pre- and post-surgery) were collected. The relative expression levels of mir-21,mir-671-5p,mir-937-3p and mir-611 in serum were quantified using quantitative real-time PCR.

Results

Our results showed that comparing preoperative and postoperative 3 months,patients'postoperative serum miR-21 decreased 0.2584-fold (95% CI:0.05664 ~0.4601),miR-671-5p decreased 0.2213-fold (95% CI:0.02188 ~0.4207),miR-937-3p decreased 0.2182-fold (95% CI:0.05234~0.3840) and miR-611 decreased 0.6224-fold (95% CI:0.4426~0.8021),and the expression levels of the above molecules were significantly lower than those before surgery (P=0.0039,0.0154,0.0031,<0.0001). Receiver operating characteristic curve (ROC) analysis showed that miR-21 (AUC=0.6942,P =0.0004) and miR-937-3p (AUC=0.6522,P =0.0059) were able to differentiate with relatively high accuracy between the preoperative and postoperative levels in early-NSCLC patients. However,no significant correlation was found between the serum levels of the studied genes and the clinical characteristics of the patients.

Conclusion

Serum miR-21 and miR-937-3p are expected to be potential biomarkers for diagnosis and prognosis in early-NSCLC patients.

表1 miRNA 引物信息
图1 NSCLC 患者术前/术后miR-21、miR-671-5p、miR-937-3p 和miR-611 表达水平
图2 NSCLC 患者中术前miR-21、miR-671-5p、miR-937-3p 和miR-611 与术后对比的ROC 曲线分析结果
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