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中华肺部疾病杂志(电子版)

中华肺部疾病杂志(电子版) ›› 2017, Vol. 10 ›› Issue (05) : 534 -538. doi: 10.3877/cma.j.issn.1674-6902.2017.05.006

所属专题： 文献；

论著

芒果苷脂质体的制备及其在Lewis肺癌移植瘤中的抗肿瘤作用及临床意义
李霞1, 吴小程1, 周人杰1,†()   
  1. 1. 400037　重庆，第三军医大学新桥医院急诊科
  • 收稿日期:2017-04-25 出版日期:2017-10-20
  • 通信作者: 周人杰
  • 基金资助:
    国家自然科学基金资助项目(30972964)

Preparation of mangiferin liposome and its antitumor effect on transplanted Lewis lung carcinoma and its clinical significance

Xia Li1, Xiaocheng Wu1, Renjie Zhou1,†()   

  1. 1. Emergency Department, Xinqiao Hosptial, Third Military Medical University, Chongqing 400037, China
  • Received:2017-04-25 Published:2017-10-20
  • Corresponding author: Renjie Zhou
  • About author:
    Corresponding author: Zhou Renjie, Email:
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李霞, 吴小程, 周人杰. 芒果苷脂质体的制备及其在Lewis肺癌移植瘤中的抗肿瘤作用及临床意义[J]. 中华肺部疾病杂志(电子版), 2017, 10(05): 534-538.

Xia Li, Xiaocheng Wu, Renjie Zhou. Preparation of mangiferin liposome and its antitumor effect on transplanted Lewis lung carcinoma and its clinical significance[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2017, 10(05): 534-538.

目的

探讨水溶性芒果苷脂质体对Lewis肺癌的治疗作用。

方法

采用薄膜法制备芒果苷脂质体，并对其形态粒径进行表征。MTT法测定其对Lewis肺癌细胞的抗增殖作用；流式细胞术测定其对Lewis肺癌细胞凋亡作用的影响；建立小鼠Lewis肺癌移植瘤模型，检测芒果苷脂质体体内抗肿瘤效果并采用。TUNEL法原位测定其对凋亡的影响。

结果

芒果苷脂质体为均匀的球状或近球状，平均粒径为(207.2±2)nm。MTT实验结果显示，芒果苷脂质体对Lewis肺癌细胞抑制作用显著高于游离芒果苷(P<0.05)，与Lewis肺癌细胞作用12 h后芒果苷IC50为(45.2±5.1)μg/ml，芒果苷脂质体IC50为(32.7±4.6)μg/ml。作用24 h后，芒果苷IC50为(22.0±3.9)μg/ml，芒果苷脂质体IC50为14.8±2.7 μg/ml。而芒果苷脂质体在体内动物实验中同样表现出抗肿瘤活力，结果显示通过小鼠尾静脉给药后，可显著抑制Lewis移植瘤在小鼠体内的生长并且与对照组相比可延长生存期超过3 d。同时，流式细胞术分析Lewis细胞凋亡实验及TUNEL法原位检测肿瘤组织细胞凋亡结果均显示芒果苷脂质体可以诱导Lewis肺癌细胞凋亡。

结论

芒果苷脂质体可用于静脉注射改善芒果苷水溶性问题，同时提高其抗肿瘤效应。

关键词: 芒果苷, 脂质体, Lewis肺癌, 细胞增殖, 细胞凋亡
Objective

To prepare water soluble mangiferin liposome and observe its effect on inhibiting the growth of transplanting Lewis lung carcinoma.

Method

Mangiferin liposome was prepared by film dispersion methodo. The shape, particles size of the liposome was characterized. Anti-proliferation and apoptosis effects induced by mangiferin liposome on the Lewis lung carcinoma cells were determined by MTT method and flow cytometric analysis in vitro. The model of mice carried Lewis lung carcinoma was established. The effect of mangiferin liposome on anti-tumor and apoptosis in vivo was investigated.

Result

The result of electromicrography showed that the liposomes consisted of small spherical or similar to spherical. The average diameter of mangiferin liposome was 207.2±2 nm. Meanwhile, the results of MTT indicated that Mangiferin liposome could more effective inhibit the proliferation of Lewis lung carcinoma than free Mangiferin (P<0.05). The IC50 of Mangiferin liposome is 32.7±4.6 μg/ml on 12 h after Mangiferin treatment and the IC50 of free Mangiferin is 45.2±5.1 μg/ml on the same time point. 24 hours later, the IC50 of Mangiferin liposome is14.8±2.7 μg/ml. In contrast, the IC50 of free Mangiferin is 22.0±3.9 μg/ml. The results in vivo also exhibited the anti-tumor effect of Mangiferin liposmes. Mangiferin liposmes could suppressed tumor growth efficiently and prolong the survival time more than 3days as control group. Furthermore, both flow cytometry of Lewis cell and TUNEL assay indiacated that mangiferin liposomes could induce apoptosis of Lewis lung carcinoma cells both in vitro and in vivo in our studies.

Conclusion

Liposome delivery system could effectively improve water solubility of mangiferin, and promote the anti-tumor effect.

Key words: Mangiferin, Liposome, Lewis lung carcinoma, Proliferation, Apoptosis
图1 芒果苷脂质体粒径测定及形态学研究；注：A：芒果苷脂质体透射电镜照片(×30 000)；B：芒果苷脂质体粒径测定结果
图2 芒果苷脂质体对Lewis肺癌细胞生长的抑制作用；注：A：芒果苷结构式；B：芒果苷脂质体及芒果苷在12或24 h时IC50；C：12 h时芒果苷及芒果苷脂质体对Lewis肺癌细胞抑制率；D：24 h时芒果苷及芒果苷脂质体对Lewis肺癌细胞抑制率(*P<0.05)
图3 芒果苷脂质体对Lewis肺癌细胞周期的影响；注：A：不同细胞周期细胞百分比；B：流式细胞仪检测不同组Lewis肺癌细胞细胞周期情况
图4 芒果苷脂质体对Lewis肺癌体内抑制情况；注：A：生存期；B：治疗后肿瘤体积变化
图5 芒果苷脂质体原位诱导凋亡情况；注：A：模型组；B：芒果苷脂质体组
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