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中华肺部疾病杂志(电子版) ›› 2019, Vol. 12 ›› Issue (01) : 38 -42. doi: 10.3877/cma.j.issn.1674-6902.2019.01.007

所属专题: 文献

论著

lncRNA PACER促进脓毒症急性肺损伤炎症反应的实验研究
史柳嫣1, 辛伟2, 位全芳3, 甘志新4, 王丹4, 胡明冬4,()   
  1. 1. 721006 宝鸡,西安医学院附属宝鸡医院科教科
    2. 721001 宝鸡,陕西省宝鸡市中医院胸外科
    3. 400038 重庆,陆军军医大学(第三军医大学)生物医学分析测试中心
    4. 400037 重庆,陆军军医大学(第三军医大学)新桥医院呼吸与危重症医学科
  • 收稿日期:2018-12-07 出版日期:2019-02-20
  • 通信作者: 胡明冬
  • 基金资助:
    国家自然科学基金资助项目(81873421); 军事医学创新工程专项(18CXZ002); 重庆市自然科学基金(cstc2017jcyjAX0335)

Experimental study of lncRNA PACER promoting inflammatory response of acute lung injury induced by sepsis

Liuyan Shi1, Wei Xin2, Quanfang Wei3, Zhixin Gan4, Dan Wang4, Mingdong Hu4,()   

  1. 1. Department of Scientific Research and Education, The Affiliated Baoji Hospital, Xi′an Medical University, Baoji 721006, China
    2. Department of Thoracic Surgery, Baoji Traditional Chinese Medicine Hospital, Baoji 721001, China
    3. Biomedical Analysis Center, Army Medical University, Chongqing 400038, China
    4. Department of Respiratory and Critical Care Medicine, XinQiao Hospital, Army Medical University, Chongqing 400037, China
  • Received:2018-12-07 Published:2019-02-20
  • Corresponding author: Mingdong Hu
  • About author:
    Corresponding author: Hu Mingdong, Email:
引用本文:

史柳嫣, 辛伟, 位全芳, 甘志新, 王丹, 胡明冬. lncRNA PACER促进脓毒症急性肺损伤炎症反应的实验研究[J]. 中华肺部疾病杂志(电子版), 2019, 12(01): 38-42.

Liuyan Shi, Wei Xin, Quanfang Wei, Zhixin Gan, Dan Wang, Mingdong Hu. Experimental study of lncRNA PACER promoting inflammatory response of acute lung injury induced by sepsis[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2019, 12(01): 38-42.

目的

探讨lncRNA PACER对小鼠脓毒症急性肺损伤炎症反应的促进作用。

方法

分离和培养急性肺损伤和健康非吸烟者的肺泡巨噬细胞,以及获取细菌脂多糖(LPS)诱导的急性肺损伤(ALI)小鼠肺组织,采用qRT-PCR方法检测lncRNA PACER的表达;过表达或敲低PACER后,ELISA方法检测THP-1和RAW264.7细胞炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的表达;对LPS所致ALI小鼠,尾静脉注射PACER siRNA慢病毒后,ELISA检测小鼠血清炎性因子TNF-α、IL-6的表达,HE染色观察肺组织病理学变化。

结果

ALI患者肺泡巨噬细胞和ALI小鼠肺组织中lncRNA PACER表达均显著升高(P<0.01);细胞过表达PACER后,细胞炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的表达升高,而敲低PACER后,炎性因子TNF-α、IL-6的表达则降低(P<0.01);ALI小鼠敲低PACER后,小鼠肺组织和血清中炎性因子TNF-α、IL-6的表达均显著降低(P<0.01),肺组织损伤程度明显减弱。

结论

lncRNA PACER可显著促进脓毒症急性肺损伤炎症反应,为明确lncRNA PACER作为ALI防治的靶标提供了依据。

Objective

To investigate the effect of lncRNA PACER on inflammatory response of acute lung injury induced by sepsis in mice.

Methods

Human alveolar macrophages were collected from 20 cases of acute lung injury and health nonsmokers, and Lung tissues of ALI mice induced by LPS were obtained to detect the expression of lncRNA PACER by qRT-PCR. Then, lncRNA PACER was overexpressed or knocked down in THP-1 and RAW264.7 cells, the expressions of cytoinflammatory cytokines TNF-α and IL-6 were detected by ELISA. Furthermore, the ALI mice induced by LPS were injected PACER siRNA lentivirus into the tail vein, and the expressions of serum inflammatory factors TNF-α and IL-6 were detected by ELISA, meanwhile, the pathologic injury of lung tissue were observed by HE staining.

Results

The expression of lncRNA PACER was significantly increased in both alveolar macrophages of ALI patients and in lung tissues of ALI mice (P<0.01). After overexpression of PACER, the expression of inflammatory cytokines TNF-α and IL-6 were increased, while the expression of inflammatory cytokines TNF-α and IL-6 decreased after knocked down PACER (P<0.01). The expression of inflammatory cytokines TNF-α and IL-6 in lung tissue and serum of ALI mice were significantly decreased after the mice were knocked down PACER (P<0.01), and the pathological damage in lung tissues was significantly alleviated in PACER knockdown mice.

Conclusions

LncRNA PACER significantly promotes the inflammatory response of acute lung injury in sepsis, which provides basis for the identification of lncRNA PACER as a target for the prevention and treatment of ALI.

图1 lncRNA PACER在ALI患者(A)和小鼠(B)模型中的表达;与Control组比较,**P<0.01
图2 lncRNA PACER促进细胞炎症因子的表达结果;注:与NC,Control组比较,**P<0.01
图3 敲低lncRNA PACER减弱ALI小鼠炎症反应(HE×100);注:与Control组比较,**P<0.01
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