探讨呼吸道病原体核酸环介导恒温扩增芯片法 (LAMP) 十三联检在下呼吸道感染常见病原体基因诊断中的价值。

通过支气管镜下吸痰收集疑似下呼吸道感染患者的深部痰液样本266例，采用LAMP技术、分离病原体并培养检测病原体，比较病原体检出率，分析呼吸道病原体LAMP十三联检在下呼吸道感染中的价值。

①266例痰液标本采用LAMP技术，检出呼吸道病原体阳性标本155例，总阳性率为58.27%，其中单种病原体感染90例(33.83%)，混合感染66例(24.81%)；共检出11种呼吸道病原体，其中检出阳性率前三位的病原体为鲍曼不动杆菌(21.05%)、耐甲氧西林金黄色葡萄球菌(20.30%)、铜绿假单胞菌(12.03%)；②257例标本同时采用病原体分离与培养技术，共92例标本培养阳性，阳性率35.80%，其中鲍曼不动杆菌44例(47.83%)，铜绿假单胞菌23例(25.00%)，肺炎克雷伯菌8例(8.70%)，肺炎链球菌和金黄色葡萄球菌未培养出。

呼吸道病原体LAMP十三联检具有高效、快速、灵敏度高、覆盖面广的优点，能为临床诊断和精准治疗提供快速可靠的实验室诊断依据。

To investigate the clinical values of the loop-mediated amplification (LAMP) chip in the gene diagnosis of common pathogens in the lower respiratory infections.

A total of 266 deep sputum samples from the lower respiratory tract were collected from the patients with suspected lower respiratory infection by fiber bronchoscopy. The pathogens were tested by LAMP or traditional isolation and cultivation. Finally, the relevance ratio of the pathogens and the values of the LAMP in the lower respiratory infections were analyzed.

Positive results of the pathogens detected by LAMP were found in 155 samples, with the positive ratio of 58.27%. Among them, single pathogen infection was found in 90 cases (33.83%) and mixed infections were found in 65 cases (24.44%). Beyond that, 11 kinds of respiratory pathogens were checked out and the top three were Acinetobacter baumannii (21.05%), Methicillin-resistant Staphylococcus (MASA, 20.30%) and Pseudomonas aeruginosa (12.03%). At the same time, traditional isolation and cultivation was adopted in 257 samples, among which, 92 samples were cultivated to be positive, with the positive ratio of 35.8%. In details, Acinetobacter baumannii was found in 44 samples (47.83%), Pseudomonas aeruginosa in 23 samples (25%) and Klebsiella pneumoniae in 8 samples. However, the Pneumonia streptococcus and Staphylococcus aureus were cultivated to be negative.

The LAMP has the advantages of rapidity, sensitivity, high efficiency and specificity, and wide coverage of the pathogens, which could be taken as a quick and reliable laboratory diagnosis basis for clinical application.