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中华肺部疾病杂志(电子版) ›› 2021, Vol. 14 ›› Issue (04) : 412 -416. doi: 10.3877/cma.j.issn.1674-6902.2021.04.002

论著

NIX蛋白表达与非小细胞肺癌临床病理特征及预后
陈燏1, 王玉秀2, 闵凌峰2,()   
  1. 1. 214500 靖江,江苏省靖江人民医院
    2. 225001 扬州,江苏省苏北人民医院·大连医科大学第二临床学院·扬州大学临床医学院
  • 收稿日期:2021-01-08 出版日期:2021-08-25
  • 通信作者: 闵凌峰
  • 基金资助:
    国家自然科学基金资助项目(81870033); 江苏省"六大人才高峰计划"(2015-WSN-106); 江苏省医学科学研究基金(QNRC2016340); 扬州市"十三五"高层次人才科研基金(ZDRC201866)

Nip-like protein Xis overexpressed and predicts poor prognosis in non-small cell lung cancer

Yu Chen1, Yuxiu Wang2, Lingfeng Min2,()   

  1. 1. Department of Respiratory Medicine, Jingjiang People′s Hospital, Yangzhou University, Jingjiang 214500, China
    2. Department of Respiratory of Subei People′s Hospital, The Second Clinical Medical College of Dalian Medical University, Clinical Medical College of Yangzhou University, Yangzhou, 225001, China
  • Received:2021-01-08 Published:2021-08-25
  • Corresponding author: Lingfeng Min
引用本文:

陈燏, 王玉秀, 闵凌峰. NIX蛋白表达与非小细胞肺癌临床病理特征及预后[J]. 中华肺部疾病杂志(电子版), 2021, 14(04): 412-416.

Yu Chen, Yuxiu Wang, Lingfeng Min. Nip-like protein Xis overexpressed and predicts poor prognosis in non-small cell lung cancer[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2021, 14(04): 412-416.

目的

探讨Nip样蛋白(Nip-like protein X,NIX/BNIP3L)在非小细胞肺癌(non-small cell lung cancer, NSCLC)中的表达情况及其临床特征和预后中的影响。

方法

选择在江苏省靖江市人民医院就诊首次确诊为NSCLC并行手术治疗的68例患者的癌组织、癌旁组织及其临床特征信息,用免疫组化法检测癌组织以及癌旁组织NIX表达情况,分析NIX表达情况与患者临床病理特征之间的相关性及对其预后的影响。

结果

免疫组化结果显示NIX在NSCLC组织表达较癌旁组织明显上调,并且NIX表达差异与NSCLC患者分化程度、淋巴结转移程度存在明显的相关性(P=0.001,P=0.005),而与患者性别、年龄、病理类型、远处转移、TNM分期无明显的相关性。多因素Cox回归分析显示NIX蛋白表达、区域淋巴结转移、病理分化程度、吸烟状况可作为非小细胞肺癌患者的独立预后因子。基于NIX在NACLC表达差异,K-M分析结果显示NIX表达的上调与患者总体生存率以及无进展生存率的下降存在明显的相关性(P<0.01)。

结论

NIX在NSCLC中的表达明显上调,NIX的表达上调可能与NSCLC的侵袭转移存在一定的相关性,NIX的表达上调可影响NSCKLC患者的预后,NIX有望成为NSCLC的治疗一新靶点。

Objective

To investigate the expression and clinical significance of Nip-like protein X (NIX/BNIP3L) in non-small cell lung cancer (NSCLC).

Methods

It was collected that tumor and adjacent tissue samples from 68 patients with NSCLC, and conducted immunohistochemistry to detect NIX expression. The correlations between NIX expression and clinicopathologic characteristics and prognosis were evaluated using the SPSS 22.0 software package.

Results

NIX was highly expressed in NSCLC tissue samples. NIX expression was correlated with the degree of NSCLC differentiation and lymph node metastasis(P=0.001, P=0.005), but not with sex, age, pathologic type, distant metastasis, or TNM stage. In addition, multivariate Cox regression analysis showed that NIX protein expression, regional lymph node metastasis, pathological differentiation, and smoking status were independent prognostic factors for NSCLC patients.Based on the differences of NIX expression in NACLC, TheK-M analysis results showed that the upregulation of NIX expression was significantly correlated with the decrease of overall survival rate and progression-free survival rateinNSCLC(P<0.01).

Conclusion

NIX expression is elevated in NSCLC and may contribute to the invasion and metastasis of lung cancer. Upregulation of NIX expression can affect the prognosis of NSCLC patients.NIX may represent a therapeutic target for the treatment of NSCLC.

图1 NSCLC癌组织中NIX的表达情况;注:NIX在肺癌组织中的表达位于细胞质;左侧标本为NIX低表达,右侧标本为NIX高表达;上方为100×镜下显示结果,下方为400×镜下显示结果
表1 NSCLC患者组织中NIX的表达与患者临床病理特征的关系
表2 临床指标Cox回归分析
图2 K-M分析;注:NSCLC组织中NIX表达与NSCLC患者的疾病总生存期(OS)/无进展期(PFS)相关;NIX低表达者OS/PFS优于高表达者(P<0.01)
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