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中华肺部疾病杂志(电子版) ›› 2022, Vol. 15 ›› Issue (04) : 481 -485. doi: 10.3877/cma.j.issn.1674-6902.2022.04.006

论著

VitD在降低PM2.5对肺泡上皮细胞毒性中的影响分析
黄婧1, 王蕾2,()   
  1. 1. 710061 西安,西安交通大学第一附属医院风湿免疫科
    2. 710004 西安,西安交通大学第二附属医院呼吸与危重症科
  • 收稿日期:2022-03-05 出版日期:2022-08-25
  • 通信作者: 王蕾
  • 基金资助:
    陕西省自然科学基础研究计划(2022JQ-761); 中国博士后科学基金会(2021M702610); 中央高校基本科研业务费(xzy012020060)

Effect of VitD on reducing the cytotoxicity of PM2.5 to alveolar epithelial cells

Jing Huang1, Lei Wang2,()   

  1. 1. Department of Rheumatism and Immunology, First Affiliated Hospital, Xi′an Jiaotong University, Xi′an, 710061, China
    2. Department of Pulmonary and Critical Care Medicine, Second Affiliated Hospital, Xi′an Jiaotong University, Xi′an, 710004, China
  • Received:2022-03-05 Published:2022-08-25
  • Corresponding author: Lei Wang
引用本文:

黄婧, 王蕾. VitD在降低PM2.5对肺泡上皮细胞毒性中的影响分析[J]. 中华肺部疾病杂志(电子版), 2022, 15(04): 481-485.

Jing Huang, Lei Wang. Effect of VitD on reducing the cytotoxicity of PM2.5 to alveolar epithelial cells[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2022, 15(04): 481-485.

目的

分析维生素D(vitamin D, VitD)在降低PM2.5对肺泡上皮细胞毒性中的影响。

方法

采用透射电子显微镜和激光诱导荧光分析对比VitD处理前后,PM2.5的毒性能力。MMT观察VitD处理前后细胞生存率,分析PM2.5对A549的毒性影响。

结果

VitD作用后的PM2.5平均粒径减小5.5 nm,颗粒聚结和团聚更为明显,颗粒平均条纹长度增加、弯曲度减小,差异有统计学意义。PM2.5条纹间距无统计学差异,有明显减小,接近0.06 nm。VitD溶液从PM2.5解吸3环和4环多环芳香烃(polycyclic aromatic hydrocarbons, PAHs),减少PM2.5表面附着的致病性PAHs。PM2.5可引起肺上皮细胞A549生存率明显下降,给予VitD干预后,PM2.5对A549细胞生存率的抑制改善了54.7%,PM2.5的生物毒性降低。

结论

VitD可减少PM2.5上吸附的致病性PAHs,抑制其致病活性,减小PM2.5对肺上皮细胞的毒性。

Objective

To investigate the decreased effect of vitamin D (VitD) on the cytotoxicity of alveolar epithelial cells by PM2.5.

Methods

Comparison of PM2.5 toxicity between without and with VitD treatment by transmission electron microscopy and laser induced fluorescence. MTT observed the effect of PM2.5 on the survival rate of A549 cells before and after VitD treatment.

Results

After the treatment of VitD, the average diameter of PM2.5 was decreased 5.5 nm. The particle agglomeration and coalescence were more obvious. The average fringe length increased and fringe tortuosity decreased, the statistics difference was significant. There was no statistical difference in the fringe spacing of particle, but there was a significant reduction, which was close to 0.06 nm. VitD solution desorbed 3-ring and 4-ring PAHs from PM2.5, reducing pathogenic PAHs on PM2.5. PM2.5 can cause a significant lung epithelial cells A549 cell death, and this effect is significantly reversed by 54.7% after VitD intervention, indicating that the biotoxicity of PM2.5 is reduced by VitD.

Conclusion

VitD can strip the pathogenic PAHs from PM2.5. Then it can significantly inhibit the pathogenic activity of PM2.5 and reduce the cytotoxicity of PM2.5.

图1 颗粒聚集形态与平均粒径统计结果对比
图2 PM2.5颗粒纳米结构参数(条纹长度、弯曲度和层间距)对比
图3 PM2.5不同作用时间VitD溶液荧光光谱比较
图4 VitD对PM2.5刺激下A549生存率的影响
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