目的

分析miR-150-5p 在青海地区结核分枝杆菌感染者血清中表达及与巨噬细胞极化关系。

方法

选择2022 年8 月至2023 年8 月青海大学附属医院、青海省第四人民医院经临床确诊的活动期肺结核（active lung tuberculosis，ALTB）35 例为观察组，结核潜伏感染（latent tuberculosis infection，LTBI）26 例为对照组，采用ELISA 法检测血清中白细胞介素10（interleukin-10，IL-10）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）；采用流式细胞术检测CD80、CD206 表达；采用qRT-PCR 试验检测miR-150-5p 表达。

结果

观察组miR-150-5p（28.11±5.41）、TNF-α（19.91±2.95）、iNOS（40.00±4.32）、CD-80（30.02±4.19）表达低于对照组miR-150-5p（38.37±3.51）、TNF-α（31.24±3.17）、iNOS（46.74±3.75）、CD-80（40.65±2.88）（P＜0.05）；观察组IL-10（100.92±5.83、81.05±9.34）、CD-206（56.33±6.10、33.72±7.65）表达高于对照组IL-10（81.05±9.34）、CD-206（33.72±7.65）（P＝0.000）。 观察组miR-150-5p 表达与IL-10 呈正相关（r＝0.385，P＜0.05），与TNF-α、iNOS 无关；对照组miR-150-5p 表达与TNF、iNOS 呈正相关（r ＝0.558，P＜0.05；r ＝0.509，P＜0.05），与IL-10 不相关（r＝-0.194，P＞0.05）。 观察组miR-150-5p 表达与CD80 呈负相关（r ＝-0.395，P＜0.05），与CD206 呈正相关（r＝0.457， P＜0.05）；对照组miR-150-5p 表达与CD80 呈正相关（r ＝0.475，P＜0.05），与CD206 呈负相关（r＝-0.614， P＜0.05）。

结论

miR-150-5p 在ALTB、LTBI 呈高表达，可为结核早期感染生物标志物。 结核感染状态下炎症因子TNF-α、IL-10、iNOS 高表达，ALTB、LTBI 中差异性表达可判断肺结核病情及疗效。 结核潜伏感染巨噬细胞向M1 型极化，活动性结核中巨噬细胞向M2 型极化。

Objective

To analyze the expression of miR-150-5p in the serum of Mycobacillus tuberculosis patients in Qinghai Province and its relationship with macrophage polarization.

Methods

Active lung tuberculosis patients diagnosed by clinical diagnosis in the Affiliated Hospital of Qinghai University and the Fourth People′s Hospital of Qinghai Province from August 2022 to August 2023 were selected.35 cases of tuberculosis （ALTB） were treated as observation group and 26 cases of latent tuberculosis infection （LTBI） as control group.Serum interleukin-10 （IL-10），tumor necrosis factor-α （TNF-α），inducible nitric oxide synthase（iNOS） were detected by ELISA； The expressions of CD80 and CD206 were detected by flow cytometry.The expression of miR-150-5p was detected by qRT-PCR assay.

Results

The expressions of miR-150-5p （28.11±5.41），TNF-α （19.91±2.95），iNOS （40.00±4.32），CD80 （30.02±4.19） in observation group were lower than those in control group （38.37±3.51） and TNF-α （31）.24±3.17），iNOS （46.74±3.75），CD80 （40.65±2.88） （P＜0.05）； The expressions of IL-10 （100.92±5.83，81.05±9.34） and CD206 （56.33±6.10，33.72±7.65） in observation group were higher than those in control group （81.05±9.34） and CD206 （33.72±7.65）（P＝0.000）.The expression of miR-150-5p in the observation group was positively correlated with IL-10 （r ＝0.385， P＜0.05），but had no correlation with TNF-α and iNOS.In control group，the expression of miR-150-5p was positively correlated with TNF and iNOS （r＝0.558， P＜0.05； r＝0.509， P＜0.05），was not correlated with IL-10 （r＝-0.194，P＞0.05）.In the observation group，the expression of miR-150-5p was negatively correlated with CD80 （r＝-0.395，P＜0.05），and positively correlated with CD206 （r ＝0.457， P＜0.05）.In the control group，the expression of miR-150-5p was positively correlated with CD80 （r＝0.475，P＜0.05），and negatively correlated with CD206 （r ＝-0.614， P＜0.05）.

Conclusion

miR-150-5p is highly expressed in ALTB and LTBI，and may be a biomarker of early tuberculosis infection.In the state of tuberculosis infection，the expression of inflammatory factors TNF-α，IL-10 and iNOS is high，and the differential expression of ALTB and LTBI can judge the condition and curative effect of tuberculosis.Macrophages in latent tuberculosis infection were polarized to M1 type，and in active tuberculosis macrophages were polarized to M2 type.