Abstract:
Objective To evaluate the value of protein chip technique in the diagnosis of tuberculosis by comparative analysis.
Methods According to lesion distribution, a total of 655 patients with tuberculosis were divided into three groups: pulmonary tuberculosis group, extrapulmonary tuberculosis group, and pulmonary&extrapulmonary tuberculosis group, with non-tuberculosis patients as the control group. Protein chip technique, TB-interferon gamma release assay (TB-IGRA), PCR assay for TB DNA(TB-PCR), bacteria-collecting smear method and bacterial culture method were applied to synchronous parallel detection for each group of samples. The positive detection rate, sensitivity and specificity of protein chip technique were compared with those of other 4 kinds of methods.
Results The results showed that the positive rates of the 5 detection methods in tuberculosis group were significantly higher than those in non-tuberculosis control group. control group, 66.26% vs. 16.53% (protein chip technique); 91.30% vs. 10.08% (TB-IGRA); 67.02% vs. 30.24% (TB-PCR); 25.04% vs. 0% (bacteria-collecting smear method); and 25.65% vs. 0% (bacterial culture method). The differences were statistically significant (P<0.05). The positive rate and sensitivity of the detection of tuberculosis by protein chip technique was significantly higher than that of bacteria-collecting smear method and culture method, and the positive rate of TB detection was similar to that of TB-PCR, which was significantly lower than that of TB-IGRA. The specificity and positive predictive value of protein chip technique were significantly higher than those of TB-PCR, significantly lower than those of bacteria-collecting smear method and bacterial culture. As compared with TB-IGRA, protein chip technique was similar in specificity, positive predictive value was significantly lower. The negative predictive value of protein chip technique was significantly higher than that of TB-PCR, smear and bacterial culture, significantly lower than that of TB-IGRA, and the differences were statistically significant (P<0.05).
Conclusion As the diagnostic techniques of tuberculosis with higher sensitivity and higher specificity, protein chip technique and TB-IGRA have complementary advantages and a good clinical application value.
Key words:
Tuberculosis,
Protein chip,
Interferon gamma release assay,
Diagnosis value
Yingkai Feng, Yong Wang, Zhenggu Huang, Xiaoli Zhao, Ying Liu. Comparative analysis of clinical values of protein chip technique and other 4 detection methods for the diagnosis of tuberculosis[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2017, 10(04): 427-430.