Effects of Rab26 on the proliferation and migration of small cell lung cancer cells H446

doi:10.3877/cma.j.issn.1674-6902.2019.01.008

Abstract

Abstract:

Objective

To explore the effect of Rab26 on the Proliferation and migration of human in small cell lung cancer cells(H446).

Methods

The H446 cells were transfected with over-expression vector of Rab26, or Rab26 siRNA. Blank group was used as a negative control, respectively. After transfection 48 h, the protein level and mRNA of Rab26 were determined by RT-PCR and Western blot. Proliferation of the H446 cells was assayed by flow cytometry and the migration rate was detected by the scratch test.

Results

The transfection efficiency of Rab26 overexpression plasmid group was (76.8±4.3) % and Rab26 siRNA group was (79.5±3.57)%. After transfection 48 hours, the expression of Rab26 in overexpression group was significantly higher than control group in mRNA and protein level (P<0.05), however, the Rab siRNA group showed that the expression of Rab26 was significantly lower than control group (P<0.05). MTT assay showed that the cell viability of blank control group was (56.4±3.23)%, (100±4.31)%, Rab26 overexpression group was (42.5±3.59)%, (62.3±2.97)% and Rab26 siRNA group was (75±3.86)%, (123.31)% in 24 and 48 hours, respectively. The cell viability of Rab26 overexpression group was significantly lower than that of blank group (P<0.05), while Rab26 siRNA group was significantly higher than that of blank group (P<0.05). Scratch test showed that the cell migration rate of blank control group was (0.53±0.03)μm/min and (0.32±0.04)μm/min, Rab26 plasmid overexpression group was (0.21±0.04)μm/min and (0.22±0.04)μm/min, the Rab26 siRNA group was (0.61±0.02) μm/min, (0.42±0.03) um/min after 24 and 48 hours. The cell migration rates of Rab26 plasmid overexpression group was significantly lower than the blank group, but Rab26 siRNA group was significantly higher than the blank group (P<0.05).

Conclusions

Rab26 is associated with the cellular proliferation and migration in H446 cell. Rab26 may be a novel therapy target for the treatments of small cell lung cancer.

Key words: Rab26, Small cell lung cancer, H446 cells, Proliferation, Migration

Wei Chen, Hua Li, Xinwei Tang, Xueping Liu. Effects of Rab26 on the proliferation and migration of small cell lung cancer cells H446[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2019, 12(01): 43-48.

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Figures/Tables 4

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