Expression and clinical significance of Krebs von den Lungen-6 and T helper 1/T helper 2 cytokines in connective tissue disease-associated interstitial pneumonia

doi:10.3877/cma.j.issn.1674-6902.2020.01.004

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of Krebs von den Lungen-6 (KL-6) and T helper 1/T helper 2 (Th1/Th2) cytokines in the connective tissue disease-associated interstitial pneumonia (CTD-IP).

Methods

A total of 77 CTD-IP patients (CTD-IP group), 60 CTD patients (CTD group), and 50 healthy volunteers (healthy group) examined in our hospital from January 2017 to January 2019 were enrolled for this study. The CTD-IP group was further divided into several subgroups according to the severity of pulmonary ventilation disorder, pulmonary diffuse dysfunction, and the imaging findings of high resolution CT (HRCT). The serum KL-6, Th1 cytokine interferon-γ (IFN-γ), Th2 cytokine interleukin-4 (IL-4) and Th1 / Th2 (IFN-γ/IL-4) levels were compared within the groups and subgroups (P<0.05). Then the values of the above-mentioned serum indicators in assessing the condition of CTD-IP patients were also analyzed.

Results

In the healthy control group, CTD group and CTD-IP group, serum KL-6 and IL-4 levels had an increasing trend, meanwhile, the IFN-γ and IFN-γ/IL-4 levels had a decreasing trend, with statistical significant differences between two groups (P<0.05). In the mild, moderate, and severe pulmonary ventilation disorder subgroups, the serum KL-6 and IL-4 levels had an increasing trend, meanwhile, the IFN-γ and IFN-γ/IL-4 levels had a decreasing trend, with statistical significant differences between two subgroups (P<0.05). In the mild, moderate, and severe pulmonary diffuse dysfunction subgroups, the serum KL-6 and IL-4 levels had an increasing trend, meanwhile, the IFN-γ and IFN-γ/IL-4 levels had a decreasing trend, with statistical significant differences between two subgroups (P<0.05). In the subgroups with different HRCT imaging findings, serum KL-6, IFN-γ, IFN-γ/IL-4 levels were significantly higher in the active group than the inactive group (P<0.05), and IL-4 levels were significantly lower in the active group than the inactive group (P<0.05).

Conclusion

Serum KL-6, IFN-γ, IL-6 and IFN-γ/IL-6 levels have a good application value in reflecting pulmonary ventilation dysfunction, pulmonary diffuse dysfunction, and disease active stage in the patients with CTD-IP, and can be used as reliable serum indicators for assessing the condition of CTD-IP patients.

Key words: Krebs von den Lungen-6, T helper 1/T helper 2 cytokines, Connective tissue disease-associated interstitial pneumonia

Huanhuan Yue, Lian Li. Expression and clinical significance of Krebs von den Lungen-6 and T helper 1/T helper 2 cytokines in connective tissue disease-associated interstitial pneumonia[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(01): 18-22.

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References 31

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组　别	例数	KL-6(U/ml)	IFN-γ(pg/ml)	IL-4(pg/ml)	IFN-γ/IL-4
健康对照组	50	176.65±23.58	319.54±50.13	211.62±26.58	1.47±0.36
CTD组	60	216.58±26.51^a	246.69±53.33^a	253.46±30.16^a	0.94±0.27^a
CTD-IP组	77	544.42±54.16^ab	213.26±41.26^ab	286.65±33.67^ab	0.76±0.31^ab
F值		1 823.821	78.548	96.521	85.661
P值		0.000	0.000	0.000	0.000

组　别	例数	KL-6(U/ml)	IFN-γ(pg/ml)	IL-4(pg/ml)	IFN-γ/IL-4
健康对照组	50	176.65±23.58	319.54±50.13	211.62±26.58	1.47±0.36
CTD组	60	216.58±26.51^a	246.69±53.33^a	253.46±30.16^a	0.94±0.27^a
CTD-IP组	77	544.42±54.16^ab	213.26±41.26^ab	286.65±33.67^ab	0.76±0.31^ab
F值		1 823.821	78.548	96.521	85.661
P值		0.000	0.000	0.000	0.000

组　别	例数	KL-6(U/ml)	IFN-γ(pg/ml)	IL-4(pg/ml)	IFN-γ/IL-4
轻度	26	369.49±42.15	253.69±36.44	243.64±31.26	1.04±0.35
中度	30	523.64±55.47^a	213.67±41.25^a	267.69±25.53^a	0.82±0.25^a
重度	21	584.15±76.62^ab	153.69±25.47^ab	323.44±29.67^ab	0.56±0.17^ab
F值		87.920	53.003	51.396	20.772
P值		0.000	0.000	0.000	0.000

组　别	例数	KL-6(U/ml)	IFN-γ(pg/ml)	IL-4(pg/ml)	IFN-γ/IL-4
轻度	26	369.49±42.15	253.69±36.44	243.64±31.26	1.04±0.35
中度	30	523.64±55.47^a	213.67±41.25^a	267.69±25.53^a	0.82±0.25^a
重度	21	584.15±76.62^ab	153.69±25.47^ab	323.44±29.67^ab	0.56±0.17^ab
F值		87.920	53.003	51.396	20.772
P值		0.000	0.000	0.000	0.000

组　别	例数	KL-6(U/ml)	IFN-γ(pg/ml)	IL-4(pg/ml)	IFN-γ/IL-4
轻度	33	416.26±44.59	276.46±35.43	244.53±32.17	1.14±0.25
中度	25	544.33±46.67^a	226.69±34.56^a	271.16±29.54^a	0.84±0.21^a
重度	19	573.64±63.35^ab	173.68±23.64^ab	337.49±30.26^ab	0.53±0.14^ab
F值		65.353	67.010	61.947	56.237
P值		0.000	0.000	0.000	0.000

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