Effect of curcumine on migration and invasion of A549 cells

doi:10.3877/cma.j.issn.1674-6902.2020.03.005

Abstract

Abstract:

Objective

To explore the effect and mechanism of curcumine on the migration and invasion of A549 cells.

Methods

Trypan blue dye exclusion assay was used to determine the effect of various concentrations of curcumine on the cell death rate. The proliferation of A549 cells was detected by MTT assay. The migration and invasion of A549 cells were determined by wound scratch assay and Transwell assay, respectively. The expression levels of EGFR, E-cadherin and N-cadherin in A549 cells affected by curcumine were detected by Western blotting method.

Results

When the curcumine concentration was 0-40 μg/ml, the mortality rate of the normal lung epithelial cells was low, and the difference was not statistically significant (P>0.05), indicating that curcumine had little toxic and side effects on the normal lung epithelial cells. The MTT results showed that curcumine inhibited the proliferation of A549 cells in a concentration-dependent manner, compared with the control group, and statistical significant difference was found between the two groups (P<0.01). The cell scratch experiment showed that the migration ability of A549 cells was reduced after treatment of curcumine for 24 hours, compared with the control group, and statistical significant difference was found between the two groups (P<0.01). Transwell assay showed that the invasiveness of A549 cells was decreased with the least amount of curcumine, compared with the control group, and statistical significant difference was found between the two groups (P<0.01). Western blotting showed that the expression of epidermal growth factor receptor (EGFR, P<0.01) and N-cadherin protein were decreased (P<0.01) and the expression of E-cadherin protein was increased (P<0.01) after treatment of 40 μg/ml of curcumine on A549 cells, compared with the control group, and statistical significant difference was found between the two groups.

Conclusion

Curcumine can inhibit the proliferation, migration and invasion of A549 cells probably through down-regulating the expression of EGFR and N-cadherin and regulating the expression of E-cadherin, and therefore reversing the process of epithelial stromal transformation.

Key words: Curcumine, Bronchogenic carcinoma, A549 cells, Migration, Invasion, EMT

Dan Tan, Xiaoqin Zeng, Wei Xu, Jian Chen, Renjie Zhou. Effect of curcumine on migration and invasion of A549 cells[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(03): 314-318.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhfbjbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-6902.2020.03.005

https://zhfbjbzz.cma-cmc.com.cn/EN/Y2020/V13/I03/314

Figures/Tables 6

References 32

1	钱桂生. 肺癌不同病理类型发病率的变化情况及其原因[J/CD]. 中华肺部疾病杂志(电子版), 2011, 4(1): 1-5.
2	陈艳丽，王媛媛，张　勇，等. 中晚期非小细胞肺癌患者化疗前后T淋巴细胞亚群表达差异分析及临床意义[J/CD]. 中华肺部疾病杂志(电子版), 2020, 13(1): 13-17.
3	李帅帅，孙　军. 姜黄素对人肝癌细胞BE-7402中环氧合酶-2表达影响的研究[J]. 中国医科大学学报，2014, 43(3): 222-225.
4	张　英，李冬梅，邢　颖. 姜黄素的药理作用与载体研究进展[J]. 中国药房，2015, 26(13): 1850-1853.
5	Liu S, Wang Z, Xu B, et al. Intermittent hypoxia reduces microglia proliferation and induces DNA damage in vitro[J]. Iran J Basic Med Sci, 2016, 19(5): 497-502.
6	Juergens RA, Brahmer JR. Adjuvant treatment in non-small cell lung cancer: Where are we now？[J]. J Natl Compr Canc Netw, 2006, 4(6): 595-600.
7	李　牧，王　丽，刘海莉，等. 姜黄素通过降低一氧化氮合酶水平抑制宫颈癌HeLa细胞的侵袭和转移[J]. 南方医科大学学报，2013, 33(12): 1752-1756.
8	Tateishi M, Ishida T, Mitsudomi T, et al. Immunohistochemical evidence of autocrine growth factors in adenocarcinoma of the human lung[J]. Cancer Res, 1990, 50(21): 7077-7080.
9	Barker AJ, Gibson KH, Grdundy, et al. Studies leading to the identification of ZD1839？(IRESSA): an orally active, selective epidermal growth factor receptor tyrosine kinase inhibitor targeted to the treatment of cancer[J]. Bioorg Med Chem Lett, 2001, 11(14): 1911-1914.
10	朱丛中，刘　娟，王新允，等. 应用组织芯片法检测EGFR、COXZ在肺癌中的表达及生物学意义[J]. 中国肺癌杂志，2010, 13(2): 107-111.
11	Christiansen JJ, Rajasekaran AK. Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis[J]. Cancer Res, 2006, 66(17): 8319-8326.
12	Galera-Ruiz, Rios MJ, Gonzalez-Campora, et al. The cadherin-catenin complex in nasopharyngeal carcinoma[J]. Eur Arch Otorhinolaryngol, 2011, 268(9): 1335-1341.
13	Maseki S, Ijichi K, Tanaka H, et al. Acquisition of EMT phenotype in the gefitinib-resistant cells of a head and neck squamous cell carcinoma cell line through Akt/GSK-3β/snail signalling pathway[J]. Br J Cancer, 2012, 106(6): 1194-1204.
14	Rosano L, Cianfrocca R, Spinella F, et al. Acquisition of chemoresistance and EMT phenotype is linked with activation of the endothelin A receptor pathway in ovarian carcinoma cells[J]. Clin Cancer Res, 2011, 17(8): 2350-2360.
15	Foroni C, Broggini M, Generali D, et al. Epithelial-mesenchymal transition and breast cancer: Role, molecular mechanism and clinical impact[J]. Cancer Treat Rev, 2012, 38(6): 689-697.
16	Fu Q, Liu X, Liu Y, et al. MicroRNA-335 and-543 suppress bone metastasis in prostate cancer via targeting endothelial nitric oxide synthase[J]. Int J Mol Med, 2015, 36(5): 1417-1425.
17	Liu S, Wang Z, Xu B, et al. Intermittent hypoxia reduces microglia proliferation and induces DNA damage in vitro[J]. Iran J Basic Med Sci, 2016, 19(5): 497-502.
18	Ranjan AP, Mukerjee A, Gdowski A, et al. Curcumin-ER prolonged subcutaneous delivery for the treatment of non-small cell lung cancer[J]. JBiomed Nanotechnol, 2016, 12(4): 679-688.
19	周晶晶，郑昱辰，李明月，等. 姜黄素的药理作用研究进展[J]. 吉林医药学院学报，2016, 37(4): 304-307.
20	戴　莉，杨　炯，潘雪凯，等. 姜黄素对肺腺癌A549细胞裸鼠移植瘤的抑制作用及其机制[J]. 中华实验外科杂志，2016, 33(10): 2358-2361.
21	Chong CR, Jänne PA. The quest to overcome resistance to EGFR-targeted therapies in cancer[J]. Nature Medicine, 2013, 19(11): 1389.
22	李松霖，谭群友，王如文，等. 新型姜黄素纳米粒对Lewis肺癌细胞增殖、凋亡的影响[J]. 第三军医大学学报，2015, 37(2): 141-145.
23	赵　莹，李英姿. 姜黄素对非小细胞肺癌A459细胞增殖及CDH13甲基化状态的影响[J]. 山东医药，2017, 57(6): 9-12.
24	Siddappa G, Kulsum S, Ravindra DR, et al. Curcumin and metformin mediated chemoprevention of oral cancer is associated with inhibition of cancer stem cells[J]. Mol Carcinog, 2017, 56(11): 2446.
25	Li ZC, Zhang LM, Wang HB, et al. Curcumin inhibits lung cancer pro-gression and metastasis through induction of FOXO1[J]. Tumor Biology, 2014, 35(1): 111.
26	Lu Y, Wei C, Xi Z. Curcumin suppresses proliferation and invasion in non-small cell lung cancer by modulation of MTA1-mediated Wnt/β-catenin pathway[J]. In Vitro Cellular & Developmental Biology-Animal, 2014, 50(9): 840.
27	Jin H, Qiao F, Wang Y, et al. Curcumin inhibits cell proliferation and induces apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of PI3K/Akt signaling pathway[J]. Oncol Reports, 2015, 34(5): 2782.
28	Wang A, Wang J, Zhang S, et al. Curcumin inhibits the development of non-small cell lung cancer by inhibiting autophagy and apoptosis[J]. Exp Ther Med, 2017, 14(5): 5075.
29	张卫平，王　珏，冉　冉. 姜黄素逆转非小细胞肺癌TKI靶向药物耐药机制的研究[J]. 中国现代医生，2017, 55(25): 37-41.
30	Shishodia S, Chaturvedi MM, Aggarwal BB. Role of Curcumin in Cancer Therapy[J]. Curr Probl Cancer, 2007, 31(4): 243-305.
31	Liu S, Wang Z, Xu B, et al. Intermittent hypoxia reduces microglia proliferation and induces DNA damage in vitro[J]. Iran J Basic Med Sci, 2016, 19(5): 497-502.
32	Rivera M, Ramos Y, Rodríguez-Valentín M, et al. Targeting multiple pro-apoptotic signaling pathways with curcumin in prostate cancer cells[J]. PLoS One, 2017, 12(6): e0179587.

浓度(μg/ml)	A570	抑制率(%)
0	0.571±0.033	-
10	0.469±0.031^a	17.86
20	0.369±0.042^b	35.37
40	0.280±0.035^b	50.96
80	0.170±0.039^b	70.35

浓度(μg/ml)	A570	抑制率(%)
0	0.571±0.033	-
10	0.469±0.031^a	17.86
20	0.369±0.042^b	35.37
40	0.280±0.035^b	50.96
80	0.170±0.039^b	70.35

[1]	Miao Li, Lianhua Zhu, Peng Han, Bo Jiang, Xiang Fei. Evaluation of relationsip between vascular morphology and risk factors for hepatocellular carcinoma by high-frame-rate contrast-enhanced ultrasound [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(09): 911-915.
[2]	Sujuan Li, Wenbo Ding, Xinping Wu, Xuedong Deng. Ultrasound-related risk factors for cervical lymph node metastasis of papillary thyroid microcarcinoma with extracapsular invasion [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2023, 20(04): 455-461.
[3]	Chunying Han, Tingting Wang, Yanyan Li, Jinxia Piao. Current research status on predictors of lymphatic vascular invasion in patients with endometrial carcinoma [J]. Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition), 2023, 19(04): 403-409.
[4]	Xiongxiong Li, Chan Zhou, Ting Xu, Yu Ren, Jin Shang. Metastasis rate of newly diagnosed ductal carcinoma in situ with microinvasive axillary lymph nodes: A Meta-analysis [J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(06): 466-474.
[5]	Shengjun Zhang, Ajing Zhao, Shoubo Li, Xianghong Hao, Minli Liu. An experimental study on the promotion of colorectal cancer invasion and migration by high glucose through HGF/c-met pathway [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(01): 21-24.
[6]	Xiang Dong, Wenliang Ma, Changwei Ji, Xiaogong Li, Gutian Zhang, Weidong Gan, Hongqian Guo. A retrospective study to assess the pseudocapsule status of renal cell carcinomas and their influencing factors [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(04): 356-360.
[7]	Jiayu Zheng, Jianjie Wu, Xiaojuan Li, Hengda Zeng, Guobang Li, Jiongduan Huang, Xingqiao Wen. Expression of hsa_circ_0090923 in prostate cancer and its effect on the proliferation and migration of prostate cancer cells [J]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(03): 276-283.
[8]	Fangfang Huang, Yamei Chen, Muhong Deng, Xinhua Shi, Shaomei Shang. Risk factors analysis and prediction model of PICC-associated venous thrombosis in lung cancer patients [J]. Chinese Journal of Lung Diseases(Electronic Edition), 2023, 16(03): 324-328.
[9]	Qing Yan, Ying Liu, Feiwen Deng, Huanwei Chen. Effect of microvascular invasion on survival and prognosis of liver transplantation recipients with liver cancer [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(06): 624-629.
[10]	Xiujun Yang, Mengying Cui, Dan Zhang. Conversion therapy of Sintilimab combined with Lenvatinib for unresectable HCC: report of one case [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(05): 581-584.
[11]	Xiaodong Wang, Kai Wang, Zhao Ge. Research progress of computer vision in improvement of therapeutic effect of liver transplantation for liver cancer [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(04): 361-366.
[12]	Di Wang, Shaocheng Lyu, Jincan Huang. Pancreatic metastasis of lung adenocarcinoma with portal vein invasion: a case report [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2023, 12(04): 457-460.
[13]	Bing Lan, Huaiming Wang, Hui Wang, Bo Ma. Research progress on locally advanced colon cancer with bladder invasion [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(06): 505-511.
[14]	Yicheng Huang, Chen Lu, Sizheng Sun, Chunzhao Yu. Expression of liver-specific transcription factors FOXA2 in human stepwise metastatic colorectal carcinoma model and its significance [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(05): 396-403.
[15]	Shidong Deng, Lingzhi Liu, Dayong Guo, Chao Wang, Zhongxin Huang, Huihui Zhang. Effects of SNHG1 gene silencing on proliferation, apoptosis, migration, and ferroptosis of bladder cancer cells [J]. Chinese Journal of Clinicians(Electronic Edition), 2023, 17(07): 804-811.

Please choose a citation manager

Content to export