Effect of VitD on reducing the cytotoxicity of PM2.5 to alveolar epithelial cells

doi:10.3877/cma.j.issn.1674-6902.2022.04.006

Abstract

Abstract:

Objective

To investigate the decreased effect of vitamin D (VitD) on the cytotoxicity of alveolar epithelial cells by PM_2.5.

Methods

Comparison of PM_2.5 toxicity between without and with VitD treatment by transmission electron microscopy and laser induced fluorescence. MTT observed the effect of PM_2.5 on the survival rate of A549 cells before and after VitD treatment.

Results

After the treatment of VitD, the average diameter of PM_2.5 was decreased 5.5 nm. The particle agglomeration and coalescence were more obvious. The average fringe length increased and fringe tortuosity decreased, the statistics difference was significant. There was no statistical difference in the fringe spacing of particle, but there was a significant reduction, which was close to 0.06 nm. VitD solution desorbed 3-ring and 4-ring PAHs from PM_2.5, reducing pathogenic PAHs on PM_2.5. PM_2.5 can cause a significant lung epithelial cells A549 cell death, and this effect is significantly reversed by 54.7% after VitD intervention, indicating that the biotoxicity of PM_2.5 is reduced by VitD.

Conclusion

VitD can strip the pathogenic PAHs from PM_2.5. Then it can significantly inhibit the pathogenic activity of PM_2.5 and reduce the cytotoxicity of PM_2.5.

Key words: PM_2.5, VitaminD, Polycyclic aromatic hydrocarbons, Biological toxicity

Jing Huang, Lei Wang. Effect of VitD on reducing the cytotoxicity of PM_2.5 to alveolar epithelial cells[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2022, 15(04): 481-485.

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