Analysis of the differential expression of miRNAs in elderly patients with bacterial pneumonia using a transcriptomics-based approach

doi:10.3877/cma.j.issn.1674-6902.2025.04.005

Abstract

Abstract:

Objective

To identify the differentially expressed miRNAs between elderly patients with bacterial pneumonia and healthy elderly individuals through transcriptomic analysis, and to conduct functional enrichment and pathway enrichment analyses on these miRNAs.

Methods

A total of 40 elderly patients (the observation group) diagnosed with bacterial pneumonia and admitted to the respiratory department of our hospital from October 2023 to June 2024, and 20 healthy elderly individuals (the control group) who underwent physical examinations at the same period were selected. Transcriptome sequencing was performed on these samples to obtain the analysis data. The transcriptome data of the bacterial infection group and the healthy group were extracted for differential miRNAs expression analysis. The screening criteria for differential miRNAs were P<0.05 and |Log2FC|>1. High-confidence differential miRNAs were further selected based on the criteria of P<1×10^-8 and |Log2FC|>1. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) were used to perform enrichment analysis on the differential miRNAs.

Results

Through transcriptomic sequencing, a total of 577 differential miRNAs(P<0.05) were identified, including 405 up-regulated miRNAs and 172 down-regulated miRNAs. According to the statistical significance(P<1×10^-8 and |Log2FC|>1), 12 high-confidence differential miRNAs were selected, including ggo-miR-142_R-1、hsa-miR-140-3p_R+ 1、hsa-miR-424-3p、hsa-miR-3909、mmu-miR-25-5p_R-1、hsa-miR-548am-3p_R-1、ptr-miR-548p_L-3R+ 2、hsa-miR-548av-3p_L+ 2R-1_1、hsa-miR-548av-3p_L+ 2R-1_2、hsa-miR-503-5p_R-2_1ss21CA 、hsa-miR-143-3p_R-1、ptr-miR-142_R-1, which was down-regulated, the others were all up-regulated. GO functional enrichment showed that differential miRNAs may affect the function of immune cells by regulating the expression of transcription factors. KEGG enrichment analysis suggested that the differential miRNAs were related to metabolic pathways.

Conclusion

The high-confidence differential miRNAs identified in elderly patients with bacterial pneumonia may serve as diagnostic markers or therapeutic targets for infection. Enrichment analysis indicated that these differential miRNAs were associated with metabolic pathways and others.

Key words: Bacterial pneumonia, Transcriptomics, The elderly, miRNAs

Yawen Guo, Qiao Hu, Chaowang Huang, Jing Zhang, Dan Wang, Peng Zhao, Xiaolong Chen, Mingdong Hu. Analysis of the differential expression of miRNAs in elderly patients with bacterial pneumonia using a transcriptomics-based approach[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2025, 18(04): 521-526.

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URL: https://zhfbjbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-6902.2025.04.005

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References 44

1	中国老年医学学会呼吸病学分会．老年肺炎临床诊断与治疗专家共识(2024年版)[J]. 中华结核和呼吸杂志，2025, 48(1): 18-34.
2	徐丽丽，刘斌，席小青，等. 老年患者肺部感染病原菌分布及耐药性分析[J]. 中国病原生物学杂志，2024, 19(9): 1075-1078＋1087.
3	Henig O, Kaye KS. Bacterial Pneumonia in Older Adults[J]. Infect Dis Clin North Am, 2017, 31(4): 689-713.
4	左蓓，刘向群，崔文洁，等. 老年细菌性肺炎患者血清脂联素和瘦素变化[J]. 中华医院感染学杂志，2020, 30(4): 512-516.
5	沈宁. 老年肺炎诊断中需要关注的问题[J]. 中华结核和呼吸杂志，2025, 48(1): 4-7.
6	Thiem U, Niklaus D, Sehlhoff B, et al. C-reactive protein, severity of pneumonia and mortality in elderly, hospitalised patients with community-acquired pneumonia[J]. Age Ageing, 2009, 38(6): 693-697.
7	Zhang F, Zhou Y, Ding J. The current landscape of microRNAs (miRNAs) in bacterial pneumonia: opportunities and challenges[J]. Cell Mol Biol Lett, 2022, 27(1): 70.
8	张帆，李思洋，丁军颖，等. MicroRNA在呼吸系统炎性疾病中的研究进展[J]. 中国比较医学杂志，2021, 31(8): 108-114.
9	Chai R, Zhou C, Hu Z, et al. Diagnostic predictability of serum miR-4793-3p and miR-1180-3p expression in community-acquired pneumonia[J]. Biomark Med, 2024, 18(6): 231-241.
10	孙艺英，徐向青，张伟强. 急诊重症监护室重症肺炎病原菌分布及其耐药性和miRNA表达[J]. 中华医院感染学杂志，2024, 34(14): 2113-2117.
11	Xu F, Zheng H, Dong X, et al. miRNA expression signatures induced by pasteurella multocida infection in goats lung[J]. Sci Rep, 2024, 14(1): 19626.
12	周云，罗翠竹，彭代娇. 革兰阴性菌感染重症肺炎患者miRNA-155与NGAL和PCT及LA对预后的影响[J]. 中华医院感染学杂志，2021, 31(19): 2978-2982.
13	中华医学会呼吸病学分会. 中国成人社区获得性肺炎诊断和治疗指南(2016年版)[J]. 中华结核和呼吸杂志，2016, 39(4): 253-279.
14	王书，张颖，张杰，等. mHLA-DR表达率对高龄老年肺炎患者免疫功能的评估及判断预后[J]. 中国老年学杂志，2020, 40(1): 78-81.
15	Pourhanifeh MH, Mahjoubin-Tehran M, Shafiee A, et al. MicroRNAs and exosomes: Small molecules with big actions in multiple myeloma pathogenesis[J]. IUBMB Life, 2020, 72(3): 314-333.
16	汪兰兰，康冬梅，周剑，等. 生长激素联合肠内营养对老年重症肺炎的影响[J]. 安徽医科大学学报，2016, 51(5): 696-699.
17	刘俊生，武勇杰，原悦. 人口老龄化、公共支出与生态可持续[J]. 经济问题，2023, (4): 37-44.
18	陈丽曼，刘文静，施虹，等. 老年肺炎患者的病原菌感染特征及细胞免疫功能研究[J]. 中国病原生物学杂志，2024, 19(12): 1452-1456.
19	甄静. 头孢哌酮舒巴坦钠治疗老年细菌性肺炎的临床疗效及对患者血清炎症因子等实验室指标的影响分析[J]. 中外医疗，2021, 40(15): 4-6＋13.
20	Gebert LFR, MacRae IJ. Regulation of microRNA function in animals[J]. Nat Rev Mol Cell Biol, 2019, 20(1): 21-37.
21	Kabekkodu SP, Shukla V, Varghese VK, et al. Clustered miRNAs and their role in biological functions and diseases[J]. Biol Rev Camb Philos Soc, 2018, 93(4): 1955-1986.
22	Ghildiyal M, Zamore PD. Small silencing RNAs: an expanding universe[J]. Nat Rev Genet, 2009, 10(2): 94-108.
23	Ha M, Kim VN. Regulation of microRNA biogenesis[J]. Nat Rev Mol Cell Biol, 2014, 15(8): 509-524.
24	Li P, Li J, Chen T, et al. Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis[J]. Int J Mol Med, 2014, 33(6): 1554-1562.
25	Stolzenburg LR, Harris A. The role of microRNAs in chronic respiratory disease: recent insights[J]. Biol Chem, 2018, 399(3): 219-234.
26	湛世本，田耕，陈建平，等. Circ-0032131通过靶向miR-140-3p调控骨关节炎软骨细胞增殖及炎症因子分泌的机制研究[J]. 现代医学，2021, 49(8): 904-910.
27	Al-Modawi RN, Brinchmann JE, Karlsen TA. Multi-pathway protective effects of MicroRNAs on human chondrocytes in an in vitro model of osteoarthritis[J]. Mol Ther Nucleic Acids, 2019, 17: 776-790.
28	Meng J, Zou Y, Hou L, et al. MiR-140-3p ameliorates the inflammatory response of airway smooth muscle cells by Targeting HMGB1 to regulate the JAK2/STAT3 signaling pathway[J]. Cell J, 2022, 24(11): 673-680.
29	Elemam NM, Hasswan H, Aljaibeji H, et al. Profiling levels of serum microRNAs and soluble ACE2 in COVID-19 patients[J]. Life (Basel), 2022, 12(4): 575.
30	李鸷，吴迪，田飞，等. circ-MFN2通过miR-489-3p调控PI3K/AKT信号通路对结直肠癌细胞增殖的影响[J]. 中国老年学杂志，2025, 45(7): 1717-1724.
31	Hann J, Bueb JL, Tolle F, et al. Calcium signaling and regulation of neutrophil functions: Still a long way to go[J]. J Leukoc Biol, 2020, 107(2): 285-297.
32	Ji SY, Lee H, Hwangbo H, et al. A novel peptide oligomer of bacitracin induces M1 macrophage polarization by facilitating Ca^2＋ influx[J]. Nutrients, 2020, 12(6): 1603.
33	戴宁，王欢，孙颖. 老年肺部感染住院患者发生难辨梭菌感染的影响因素研究[J]. 中国全科医学，2019, 22(27): 3353-3356.
34	李燕燕，陶建双，徐锐. 老年冠脉搭桥术后患者衰弱状态与预后的关系[J]. 新疆医学，2025, 55(2): 146-149.
35	李瑾，张秀月，李泽，等. 2017-2023年某三甲医院老年住院患者医院感染流行病学特征[J]. 中华医院感染学杂志，2025, 35(3): 465-470.
36	赵畅，谢芳，向英. 老年重症肺炎不同血糖水平患者痰液宏基因组学与菌群相关性研究[J]. 现代检验医学杂志，2024, 39(4): 116-120.
37	张帆，宋琳，韩锋锋. 代谢组学在肺部感染性疾病中的研究进展[J]. 中国呼吸与危重监护杂志，2024, 23(10): 741-744.
38	夏世金，高文，胡明冬，等. 环状RNA的研究现状及展望[J/CD]. 中华肺部疾病杂志(电子版), 2014, 7(6): 670-673.
39	崔露，李萧，李杏，等. 基于miRNA测序技术探讨艾灸治疗骨质疏松模型大鼠的分子机制[J/OL]. 中国比较医学杂志，1-13[2025-06-06]． URL
40	姚兰，刘亚男，余追. T细胞免疫球蛋白粘蛋白-3/凝集素9通路在脓毒血症免疫调控中的研究进展[J]. 微循环学杂志，2018, 28(1): 71-74＋79.
41	边伟帅，陈炜，甄洁，等. 高通量二代测序鉴定ICU重症肺炎老年患者下呼吸道菌群变化临床意义[J]. 中国老年学杂志，2022, 42(15): 3677-3680.
42	杨兰，凌玲，杨帆，等. miR-144在β地中海贫血中的表达及作用研究[J]. 中国实验血液学杂志，2025, 33(02): 491-497.
43	王挺，张杨，郭洁，等. miR-4435对鼻咽癌细胞生物学行为的影响研究[J/OL]. 现代医药卫生，1-8[2025-04-13]． URL
44	韩超，何梦祺，徐哲荣. 老年住院患者发生吸入性肺炎的影响因素[J]. 中国老年学杂志，2021, 41(13): 2734-2737.

miR基因	miR序列	上调/下调	倍数变化	log2(倍数变化)	P值(t检验)
ggo-miR-142_R-1	CCCATAAAGTAGAAAGCACT	上调	2.05	1.03	5.37E-12
hsa-miR-140-3p_R＋1	TACCACAGGGTAGAACCACGGA	上调	2.45	1.29	3.41E-10
hsa-miR-424-3p	CAAAACGTGAGGCGCTGCTAT	上调	2.83	1.50	5.07E-10
hsa-miR-3909	TGTCCTCTAGGGCCTGCAGTCT	上调	4.41	2.14	1.89E-09
mmu-miR-25-5p_R-1	AGGCGGAGACTTGGGCAATTG	上调	4.24	2.08	2.19E-09
hsa-miR-548am-3p_R-1	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
ptr-miR-548p_L-3R＋2	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-548av-3p_L＋2R-1_1	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-548av-3p_L＋2R-1_2	CCAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-503-5p_R-2_1ss21CA	TAGCAGCGGGAACAGTTCTGA	上调	2.67	1.42	4.14E-09
hsa-miR-143-3p_R-1	TGAGATGAAGCACTGTAGCT	上调	2.49	1.32	8.08E-09
ptr-miR-142_R-1	TGTAGTGTTTCCTACTTTATGG	下调	0.49	－1.03	9.98E-09

miR基因	miR序列	上调/下调	倍数变化	log2(倍数变化)	P值(t检验)
ggo-miR-142_R-1	CCCATAAAGTAGAAAGCACT	上调	2.05	1.03	5.37E-12
hsa-miR-140-3p_R＋1	TACCACAGGGTAGAACCACGGA	上调	2.45	1.29	3.41E-10
hsa-miR-424-3p	CAAAACGTGAGGCGCTGCTAT	上调	2.83	1.50	5.07E-10
hsa-miR-3909	TGTCCTCTAGGGCCTGCAGTCT	上调	4.41	2.14	1.89E-09
mmu-miR-25-5p_R-1	AGGCGGAGACTTGGGCAATTG	上调	4.24	2.08	2.19E-09
hsa-miR-548am-3p_R-1	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
ptr-miR-548p_L-3R＋2	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-548av-3p_L＋2R-1_1	CAAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-548av-3p_L＋2R-1_2	CCAAAACTGCAGTTACTTTTG	上调	2.04	1.03	3.09E-09
hsa-miR-503-5p_R-2_1ss21CA	TAGCAGCGGGAACAGTTCTGA	上调	2.67	1.42	4.14E-09
hsa-miR-143-3p_R-1	TGAGATGAAGCACTGTAGCT	上调	2.49	1.32	8.08E-09
ptr-miR-142_R-1	TGTAGTGTTTCCTACTTTATGG	下调	0.49	－1.03	9.98E-09

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