To develop a high-performance electrochemical immunosensor for detecting the lung cancer biomarker neuron specific enolase (NSE).

Two dimensional nano material boron nitride (BN) was used as the adsorption matrix to deposit nano gold (Au), effectively increasing the specific surface area; Using molybdenum disulfide supported platinum palladium (MoS₂@PtPd) nano mimetic enzyme complex for electron transfer catalysis and catalytic amplification signal detection of NSE; Determine the optimal conditions by examining the key factors involved in constructing sensors, and conduct repeatability, stability, and specificity tests on the sensors; And conduct methodological validation on clinical specimens.

It showes that the diameter range of BN was 200~500 nm, nm, the diameter range of MoS₂ was 500~800 nm, and the MoS₂ @ PtPd complex was about 50~120 nm. The current of BN binding on the electrode surface decreased by 40 μ A, and the current increased by 15 μ A after gold deposition. The blocking current of bovine serum albumin (BSA) decreased by 9 μ A, and the capture current of NSE decreased by 15 μ A; The optimal total concentration for catalyzing H₂O₂ is 3 μ M, and the optimal binding time for the secondary antibody to bind to the antigen is after 3 hours; When detecting NSE, the standard curve is: I=-5.13-0.40 LogC_NSE, r=0.92, and the low of detection (LOD) is 0.86 pg/ml; The relative standard deviation (R.S.D.) between sensor batches was 2.91%, and after 21 days, the peak current remained at 98.14% of its initial current; Compared with other interfering proteins at a concentration of 1 ng/ml, NSE was (2.10±0.05)μA, alpha fetoprotein (AFP) was (0.12±0.03)μA, carcinoembryonic antigen (CEA) was (0.15±0.02)μA, albumin (ALB) was (0.21±0.02)μA, and ferritin (FER) was (0.19±0.03)μA (P<0.05); The relative deviation of four serum samples with severe applicability ranges from 1.70% to 4.11%, and the recovery rate ranges from 93.09% to 103.12%.

The novel immunosensor based on MoS₂ @ PtPd catalytic combined with BN/Au membrane is simple to construct and has good performance for NSE detection. It provides an alternative method for early diagnosis of lung cancer, and has clinical significance.