探讨糖尿病大鼠肺脏的NF-κB表达情况及NF-κB的表达增强对肺脏损伤的程度。

30只10周龄SD大鼠随机分为正常对照组(A组，雌雄各5只)、糖尿病模型组(B组，雌雄各10只)，采用链脲菌素诱导建立12周糖尿病大鼠模型。模型建立12周后，全部大鼠采用20%的乌拉坦1.35 g/kg腹腔内注射麻醉处死，并取左肺下叶固定切片。光学显微镜下观察肺组织炎症程度，免疫组织化学方法检测肺组织NF-κB表达的阳性面积百分比。

①肺部炎症观察：大多数正常对照组大鼠肺组织内未见炎症细胞。糖尿病模型组可见片状炎症细胞聚集，局部肺泡结构消失；②NF-κB的表达：NF-κB主要表达于气管、肺泡的上皮细胞内。正常对照组肺组织阳性表达明显弱于糖尿病模型组。分别对正常对照组和糖尿病模型组大鼠肺脏支气管上皮细胞和肺泡壁上皮细胞的NF-κB的表达做秩和检验(P<0.05)具有明显统计学差异。

2型糖尿病大鼠模型肺组织内时炎症的改变程度较正常对照组相比明显加重，NF-κB的表达增加可能是糖尿病时肺脏病变的一个重要的危险因素。

To detect the expression of NF-κB in the detection of diabetic rat lungs to study whether diabetes can lead to enhanced expression of NF-κB in the lungs resulting in lung injury of varying degrees.

30 cases of 10 weeks old SD rats were randomly divided into normal control group (group A, male and female 5), diabetic model group (B group, male and female 10) with streptozotocin induced by 12 weeks diabetic rat model. Model after 12 weeks, all rats were 20% urethane 1.35 g/kg intraperitoneal injection of anesthesia were sacrificed, and were taken to the left lower lobe. The percentage of positive area of lung tissue inflammation in the degree of immunohistochemical method for detection of lung tissue NF-κB expression was observed under an optical microscope.

① lung inflammation observed: no inflammatory cells in the lung tissue of most of the normal control rats. Diabetic model group visible sheet inflammatory cell aggregation, the local alveolar structure disappeared; ②of NF-κB expression: NF-κB was mainly expressed in the trachea, alveolar epithelial cells, the epithelial cells of the vascular wall and expression but significantly weaker than the expression on the former two. Positive expression of normal lung tissue of the control group was significantly weaker than the DM group. Respectively rank sum test (P<0.05) with significant difference between the expression of the normal control group and diabetic model rats lung bronchial epithelial cells and alveolar wall epithelial cells, NF-κB.

NF-κB expression in type 2 diabetic rat model of lung tissue significantly increased while inflammation of the degree of change compared with control group was significantly heavier, so we have come to the conclusion of type 2 diabetes so that the lungs of rats with NF-κB expression increased, the increase in the expression of NF-κB has become an important risk factor for lung lesions of varying degrees of diabetes.