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中华肺部疾病杂志(电子版) ›› 2024, Vol. 17 ›› Issue (06) : 1003 -1007. doi: 10.3877/cma.j.issn.1674-6902.2024.06.029

临床研究

哮喘患儿IL-10 基因多态性与肺功能及外周血Treg 细胞的相关性
黄波翠1, 蔡思铭1, 古裕鸟1, 庄秀娟1, 钟娇霞1, 吴小文1, 霍开明1,()   
  1. 1.570100 海口,海南医科大学第二附属医院儿科二区
  • 收稿日期:2024-07-15 出版日期:2024-12-25
  • 通信作者: 霍开明

Correlation of IL-10 gene polymorphisms with lung function and peripheral blood Treg cells in children with asthma

Bocui Huang, Siming Cai, Yuniao Gu, Xiujuan Zhuang, Jiaoxia Zhong, Xiaowen Wu, Kaiming Huo()   

  • Received:2024-07-15 Published:2024-12-25
  • Corresponding author: Kaiming Huo
引用本文:

黄波翠, 蔡思铭, 古裕鸟, 庄秀娟, 钟娇霞, 吴小文, 霍开明. 哮喘患儿IL-10 基因多态性与肺功能及外周血Treg 细胞的相关性[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(06): 1003-1007.

Bocui Huang, Siming Cai, Yuniao Gu, Xiujuan Zhuang, Jiaoxia Zhong, Xiaowen Wu, Kaiming Huo. Correlation of IL-10 gene polymorphisms with lung function and peripheral blood Treg cells in children with asthma[J/OL]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(06): 1003-1007.

目的

分析哮喘患儿白介素-10(interleukin-10,IL-10)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与肺功能及外周血调节性T 细胞(regulatory cell,Treg)水平的相关性。

方法

选取2021 年2 月至2024 年2 月我院收治的57 例哮喘患儿为对象,病情严重程度中度33 例为观察组和轻度24 例对照组,采用实时聚合酶链反应(polymerase chain reaction,PCR)对两组的4 种IL-10 基因SNP 位点进行基因分型,检测外周血IL-10 及单核细胞中Tregs 细胞水平、肺功能情况,采用Pearson相关性分析IL-10 基因多态性与肺功能及外周血Treg 细胞水平的相关性。

结果

观察组第1 秒用力呼气容积(forced expiratory volume in one second,FEV1)(27.45±3.56)% 、用力肺活量(forced vital capacity,FVC)(39.40±5.81)%及FEV1/FVC 比值(66.29±5.42)%低于对照组FEV1(31.48±2.93)%、FVC[(43.42±6.02)%及FEV1/FVC 比值(75.15±6.13)%(P<0.05);观察组IL-10 水平(94.83±11.46)pg/ml、Tregs 细胞(1.85±0.54)%低于对照组IL-10 水平(105.43±15.20)pg/ml、Tregs 细胞(2.43±0.61)%(P<0.05)。 观察组IL-10 基因rs3024491 位点的TT 基因型占比75.00%,两组IL-10 基因rs3024496、rs3024491 位点基因型的分布频率差异具有统计学意义(P<0.05);观察组不同IL-10 基因rs3024491 位点基因型分型IL-10水平、外周血Treg 细胞水平及FEV1/FVC 比值差异具有统计学意义(P<0.05);Pearson 相关性分析显示,IL-10 基因rs3024491 位点的TT 基因型与IL-10 及FEV1/FVC 比值呈正相关(r=0.508,P=0.000;r=0.379,P=0.000),与外周血Treg 细胞水平呈负相关(r=-0.446,P=0.000)。

结论

中度哮喘患儿中IL-10基因rs3024491 位点多态性频率高,TT 基因型与肺功能、IL-10 降低和Tregs 细胞增加相关,影响患儿哮喘严重程度。

表1 两组哮喘患儿肺功能结果比较[( ±s),(%)]
表2 两组哮喘患儿IL-10 水平和Tregs 细胞比较( ±s
表3 两组IL-10 基因位点多态性分布情况比较[频数/频率(%)]
表4 IL-10 基因rs3024491 位点多态性与IL-10、外周血Treg 细胞及肺功能( ±s
表5 IL-10 基因rs3024491 位点多态性与肺功能及外周血Treg 细胞水平
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