Detection and analysis of multiplex real-time polymerase chain reaction assay of pathogens in lower respiratory tract infections

doi:10.3877/cma.j.issn.1674-6902.2019.05.005

Abstract

Abstract:

Objective

To compare the detection results of the samples from the patients with lower respiratory tract infections with multiplex real-time polymerase chain reaction (PCR) assay (MRT-PCR) and indirect immunofluorescence assay (IFA).

Methods

The samples of the infected lower respiratory tract were taken from 210 patients in our hospital during January 2014 to December 2017. The samples were detected by MRT-PCR and IFA, respectively, and then received sequencing analysis. Then the sensitivity and specificity of the two kinds of assays were compared.

Results

The positive rates of the pathogens in the 210 samples for the MRT-PCR and IFA were 58.57% and 38.10%, respectively, and the mixed infection rates were 7.62% and 4.76%, respectively. The sensitivities for the MRT-PCR and IFA were 94.74% and 35.96%, respectively, and the specificities for the MRT-PCR and IFA were 84.38% and 59.38%, respectively. There were significant differences in the sensitivities and specificities of the two kinds of assays (P<0.05).

Conclusion

Compared with the IFA assay, the MRT-PCR assay has a higher sensitivity and a higher specificity in the detection of the pathogens in the lower respiratory tract infections.

Key words: Lower respiratory tract infections, Respiratory virus, Atypical pathogen, Indirect immunofluorescence assay, Multiplex real-time polymerase chain reaction

Xinyu Liu, Meng Li, Puyu Shi, Mingwei Chen. Detection and analysis of multiplex real-time polymerase chain reaction assay of pathogens in lower respiratory tract infections[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2019, 12(05): 559-562.

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References 25

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病原体	MRT-PCR								IFA
病原体	TP	FP	FN	TN	灵敏度(%)	特异度(%)	PPV(%)	NPV(%)	TP	FP	FN	TN	灵敏度(%)	特异度(%)	PPV(%)	NPV(%)
FluA	35	4	0	171	100.00	97.71	89.74	100.00	12	12	23	163	34.29	93.14	50.00	87.63
RSV	8	0	0	202	100.00	100.00	100.00	100.00	2	2	6	200	25.00	99.01	50.00	97.09
PIV	12	1	0	197	100.00	99.49	92.31	100.00	2	1	10	197	16.67	99.49	66.67	95.17
FluB	7	2	0	201	100.00	99.01	77.78	100.00	2	5	5	198	28.57	97.54	28.57	97.54
ADV	9	2	2	197	81.82	98.99	81.82	98.99	2	3	9	196	18.18	98.49	40.00	95.61
MP	40	6	3	161	93.02	96.41	86.96	98.17	17	14	26	153	39.53	91.62	54.84	85.47
CP	4	0	0	206	100.00	100.00	100.00	100.00	4	5	0	201	100.00	97.57	44.44	100.00
LP	9	0	1	200	90.00	100.00	100.00	99.50	4	3	6	197	40.00	98.50	57.14	97.04

病原体	MRT-PCR								IFA
病原体	TP	FP	FN	TN	灵敏度(%)	特异度(%)	PPV(%)	NPV(%)	TP	FP	FN	TN	灵敏度(%)	特异度(%)	PPV(%)	NPV(%)
FluA	35	4	0	171	100.00	97.71	89.74	100.00	12	12	23	163	34.29	93.14	50.00	87.63
RSV	8	0	0	202	100.00	100.00	100.00	100.00	2	2	6	200	25.00	99.01	50.00	97.09
PIV	12	1	0	197	100.00	99.49	92.31	100.00	2	1	10	197	16.67	99.49	66.67	95.17
FluB	7	2	0	201	100.00	99.01	77.78	100.00	2	5	5	198	28.57	97.54	28.57	97.54
ADV	9	2	2	197	81.82	98.99	81.82	98.99	2	3	9	196	18.18	98.49	40.00	95.61
MP	40	6	3	161	93.02	96.41	86.96	98.17	17	14	26	153	39.53	91.62	54.84	85.47
CP	4	0	0	206	100.00	100.00	100.00	100.00	4	5	0	201	100.00	97.57	44.44	100.00
LP	9	0	1	200	90.00	100.00	100.00	99.50	4	3	6	197	40.00	98.50	57.14	97.04

MRT-PCR	IFA	测序	MRT-PCR	IFA	测序	MRT-PCR	IFA	测序
FluA+MP	-	FluA+MP	PIV	-	PIV	LP	-	LP
FluA+MP	FluA	FluA+MP	PIV	-	-	-	FluA+RSV	-
FluA+RSV	-	FluA+RSV	FluB	FluB+MP	FluB	-	FluA	-
FluB+MP	-	FluB+MP	FluB	-	-	-	RSV	-
FluB+RSV	-	FluB+RSV	ADV	LP	ADV	-	FluB	-
PIV+LP	-	PIV+LP	ADV	CP	ADV	-	MP	-
FluA	MP	FluA	ADV	-	ADV	-	LP	-
FluA	LP+PIV	FluA	ADV	-	-	-	CP	-
FluA	FluA+MP	FluA	MP	-	MP	-	FluA+MP	-
FluA	-	FluA	MP	FluA	MP	-	ADV	-
FluA	-	-	MP	-	MP
RSV	FluA	RSV	MP	-	-

MRT-PCR	IFA	测序	MRT-PCR	IFA	测序	MRT-PCR	IFA	测序
FluA+MP	-	FluA+MP	PIV	-	PIV	LP	-	LP
FluA+MP	FluA	FluA+MP	PIV	-	-	-	FluA+RSV	-
FluA+RSV	-	FluA+RSV	FluB	FluB+MP	FluB	-	FluA	-
FluB+MP	-	FluB+MP	FluB	-	-	-	RSV	-
FluB+RSV	-	FluB+RSV	ADV	LP	ADV	-	FluB	-
PIV+LP	-	PIV+LP	ADV	CP	ADV	-	MP	-
FluA	MP	FluA	ADV	-	ADV	-	LP	-
FluA	LP+PIV	FluA	ADV	-	-	-	CP	-
FluA	FluA+MP	FluA	MP	-	MP	-	FluA+MP	-
FluA	-	FluA	MP	FluA	MP	-	ADV	-
FluA	-	-	MP	-	MP
RSV	FluA	RSV	MP	-	-

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