TH17 cells balance and mechanism in the immune microenvironment on the pathogenesis of allergic asthma

doi:10.3877/cma.j.issn.1674-6902.2020.05.014

Abstract

Abstract:

Objective

To investigate the T helper cells 17 and the related cytokines expression level in the role of allergic asthma pathogenesis mechanism.

Methods

This study selected 120 cases of asthma patients as the research object of this study. Depending on the type of asthma patients, 120 patients were divided into allergic asthma and allergic asthma in the two groups, each group of 60 patients, hospital medical examination at the same time; choose the same period of 48 cases of healthy people as control group. Determination of Th17 and Treg in three groups of participants were peripheral blood, Th2 ratio and serum and induced sputum IL-17A, IL-4, IL-5, IL-10 and IL-18 levels. In addition, determination of neutrophil in induced sputum three groups of patients, acidophilic granulocyte ratio.

Results

Th17 ratio in allergic asthma group was higher than that in control group (P<0.05). Treg ratio in allergic asthma group was lower than that in control group (P<0.05). Th17 ratio in allergic asthma group was lower than that in non-allergic asthma group (P<0.05). There was no significant difference in Th2 ratio among the three groups (P>0.05). The levels of IL-17A, IL-4 and IL-5 in the allergic asthma group were higher than those in the control group (P<0.05). The levels of IL-10 and IL-18 in the allergic asthma group were lower than those in the control group (P<0.05). The levels of IL-17A in the allergic asthma group were lower than those in the non-allergic asthma group (P<0.05); the levels of IL-4 in the allergic asthma group were higher than those in the non-allergic asthma group. The levels of IL-17A, IL-4, IL-5, IL-10 and IL-18 in asthma group were higher than those in control group. The levels of IL-17A and IL-18 in allergic asthma group were lower than those in non-allergic asthma group (P<0.05). The levels of IL-4 in allergic asthma group were higher than those in non-allergic asthma group (P<0.05). There was no significant difference in the levels of IL-5 and IL-10 between the two groups (P>0.05). The proportion of neutrophils and eosinophils in allergic asthma group was higher than that in control group (P<0.05). The proportion of neutrophils in allergic asthma group was lower than that in non-allergic asthma group (P<0.05). The proportion of eosinophils in allergic asthma group was higher than that in non-allergic asthma group (P<0.05).

Conclusion

TH17 and other cytokine immune imbalance is closely related to the occurrence of allergic asthma.

Key words: Allergic asthma, T helper cells 17, Immune balance, Cellular microenvironment

Li Wang, Xiaojun Wang. TH17 cells balance and mechanism in the immune microenvironment on the pathogenesis of allergic asthma[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(05): 648-652.

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References 30

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组　别		例数	指　标
组　别		例数	Th17比例	Treg比例	Th2比例
过敏性哮喘组		60	1.89±0.13	0.50±0.08	1.69±0.34
非过敏性哮喘组		60	2.08±0.15	0.38±0.07	1.63±0.26
对照组		48	0.86±0.11	1.31±0.42	0.49±0.21
	F值		38.16	27.53	23.89
	P值		<0.05	<0.05	>0.05

组　别		例数	指　标
组　别		例数	Th17比例	Treg比例	Th2比例
过敏性哮喘组		60	1.89±0.13	0.50±0.08	1.69±0.34
非过敏性哮喘组		60	2.08±0.15	0.38±0.07	1.63±0.26
对照组		48	0.86±0.11	1.31±0.42	0.49±0.21
	F值		38.16	27.53	23.89
	P值		<0.05	<0.05	>0.05

组　别		例数	指　标
组　别		例数	IL-17A	IL-4	IL-5	IL-10	IL-18
过敏性哮喘组		60	1.38±0.36	31.98±5.87	379.63±69.18	1.65±0.23	259.67±23.97
非过敏性哮喘组		60	1.78±0.36	23.99±5.12	385.63±20.16	1.59±0.17	268.13±43.17
对照组		48	0.69±0.21	20.39±3.59	331.71±20.31	7.15±0.26	266.84±53.66
	F值		38.89	31.53	35.48	46.98	30.81
	P值		<0.05	<0.05	<0.05	<0.05	<0.05

组　别		例数	指　标
组　别		例数	IL-17A	IL-4	IL-5	IL-10	IL-18
过敏性哮喘组		60	1.38±0.36	31.98±5.87	379.63±69.18	1.65±0.23	259.67±23.97
非过敏性哮喘组		60	1.78±0.36	23.99±5.12	385.63±20.16	1.59±0.17	268.13±43.17
对照组		48	0.69±0.21	20.39±3.59	331.71±20.31	7.15±0.26	266.84±53.66
	F值		38.89	31.53	35.48	46.98	30.81
	P值		<0.05	<0.05	<0.05	<0.05	<0.05

组　别		例数	指　标
组　别		例数	IL-17A	IL-4	IL-5	IL-10	IL-18
过敏性哮喘组		60	1.37±0.28	51.48±13.26	429.78±69.83	51.99±30.16	380.64±61.11
非过敏性哮喘组		60	1.87±0.87	37.99±17.53	411.17±31.65	49.82±15.58	483.63±152.66
对照组		48	0.52±0.15	18.25±2.19	229.6±14.96	5.95±0.20	202.52±45.21
	F值		48.63	47.66	28.15	58.97	31.73
	P值		<0.05	<0.05	>0.05	>0.05	<0.05

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