Therapeutic effect of "Tanshinone ⅡA+ Osthole" formula on acute lung injury in mice

doi:10.3877/cma.j.issn.1674-6902.2024.02.001

Abstract

Abstract:

Objective

To analyze the therapeutic effects of Tanshinone (ⅡA)+ Ossathol in the treatment of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.

Method

A total of 50 C57 mice were randomly divided into control group (normal saline), LPS group, Tanshinone ⅡA group, Osthole group and Tanshinone ⅡA+ Osyhole group, with 10 mice in each group. The mice acute lung injury model was established by endotracheal infusion of LPS. The survival rate and lung histopathology of mice were observed after 24 hours of the model was made. The left lung wet/dry weight ratio (W/D) and the total cell count in bronchoalveolar lavage fluid (BALF) at 72 h were detected. Total protein, myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α) and interleukin-6 (interleukin-6) and interleukin-1 (IL-1) were detected by ELISA.

Results

The 24 h survival rate of mice in LPS group was 0, the 72 h survival rate of mice in Tanshinone ⅡA group and Osthole group was 50%, and the 72 h survival rate of mice in Tanshinone ⅡA+ Osthole group was about 75%, and the survival rate was improved. The results of HE staining showed that the damage of pathological structure of lung tissue in Tanshinone ⅡA group and Osthole group was less than that in LPS group, and the repair of pathological structure of lung tissue in Tanshinone ⅡA+ Osthole group was more obvious. W/D results showed that compared with LPS group, the pulmonary edema of Tanshinone ⅡA group and Osthole group were not improved, and the pulmonary edema of Tanshinone ⅡA+ Osthole group was alleviated (P<0.05). The total protein content and cell count in BALF showed that compared with LPS group, the pulmonary protein exudation and inflammatory cell secretion were reduced in Tanshinone ⅡA group and Osthole group, and significantly decreased in Tanshinone ⅡA+ Osthole group (P<0.05). The results of MPO activity were similar to those of cell count in BALF. The secretion of pulmonary inflammatory factors TNF-α, IL-6 and IL-1 in Tanshinone ⅡA group and Osthole group were decreased compared with LPS group(P<0.05). The secretion of pulmonary inflammatory factor decreased significantly in Tanshinone ⅡA+ Osthole group (P<0.05).

Conclusion

Tanshinone ⅡA+ Osthole formula can reduce the secretion of lung inflammatory factors induced by LPS, relieve inflammatory cell infiltration, alveolar protein exudation, inflammatory cell leakage and pulmonary edema, improve lung pathological structure and increase the survival rate of mice. Tanshinone ⅡA+ Osthole formula has therapeutic effect on LPS-induced acute lung injury in mice.

Key words: Acute lung injury, Tanshinone ⅡA, Osthole, Lipopolysaccharide

Yujuan Li, Fang Ai, Huanqing Xiong, Jian Chen, Gang Liu, Zhichao Li, Faguang Jin. Therapeutic effect of "Tanshinone ⅡA+ Osthole" formula on acute lung injury in mice[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(02): 171-177.

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Figures/Tables 6

References 40

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