Abstract:

Objective

To analyze the expression of miR-150-5p in the serum of Mycobacillus tuberculosis patients in Qinghai Province and its relationship with macrophage polarization.

Methods

Active lung tuberculosis patients diagnosed by clinical diagnosis in the Affiliated Hospital of Qinghai University and the Fourth People′s Hospital of Qinghai Province from August 2022 to August 2023 were selected.35 cases of tuberculosis （ALTB） were treated as observation group and 26 cases of latent tuberculosis infection （LTBI） as control group.Serum interleukin-10 （IL-10），tumor necrosis factor-α （TNF-α），inducible nitric oxide synthase（iNOS） were detected by ELISA； The expressions of CD80 and CD206 were detected by flow cytometry.The expression of miR-150-5p was detected by qRT-PCR assay.

Results

The expressions of miR-150-5p （28.11±5.41），TNF-α （19.91±2.95），iNOS （40.00±4.32），CD80 （30.02±4.19） in observation group were lower than those in control group （38.37±3.51） and TNF-α （31）.24±3.17），iNOS （46.74±3.75），CD80 （40.65±2.88） （P＜0.05）； The expressions of IL-10 （100.92±5.83，81.05±9.34） and CD206 （56.33±6.10，33.72±7.65） in observation group were higher than those in control group （81.05±9.34） and CD206 （33.72±7.65）（P＝0.000）.The expression of miR-150-5p in the observation group was positively correlated with IL-10 （r ＝0.385， P＜0.05），but had no correlation with TNF-α and iNOS.In control group，the expression of miR-150-5p was positively correlated with TNF and iNOS （r＝0.558， P＜0.05； r＝0.509， P＜0.05），was not correlated with IL-10 （r＝-0.194，P＞0.05）.In the observation group，the expression of miR-150-5p was negatively correlated with CD80 （r＝-0.395，P＜0.05），and positively correlated with CD206 （r ＝0.457， P＜0.05）.In the control group，the expression of miR-150-5p was positively correlated with CD80 （r＝0.475，P＜0.05），and negatively correlated with CD206 （r ＝-0.614， P＜0.05）.

Conclusion

miR-150-5p is highly expressed in ALTB and LTBI，and may be a biomarker of early tuberculosis infection.In the state of tuberculosis infection，the expression of inflammatory factors TNF-α，IL-10 and iNOS is high，and the differential expression of ALTB and LTBI can judge the condition and curative effect of tuberculosis.Macrophages in latent tuberculosis infection were polarized to M1 type，and in active tuberculosis macrophages were polarized to M2 type.

Key words: Pulmonary tuberculosis, miRNA, Mycobacterium tuberculosis, Macrophage polarization