Inhibitory effect of plasma-activated physiological saline on Mycobacterium tuberculosis in vitro

doi:10.3877/cma.j.issn.1674-6902.2025.02.014

Abstract

Abstract:

Objective

To analyze the in vitro bacteriostatic effect and clinical significance of plasma activated saline （PAS） on Mycobacterium tuberculosis.

Methods

One standard strain of Mycobacterium tuberculosis and five clinically isolated mycobacterium tuberculosis （No. 6180，6943，7158，6545，7154）were selected for growth experiments. Mycobacterium tuberculosis was treated with normal saline （NS group），plasma activated saline （PAS group） and hydrochloric acid （HCl group）. The colony morphology of Mycobacterium tuberculosis in each group was observed after 31 days of culture，and the number of bacteria was counted after 14，20，26 and 31 days of culture. The growth curve was drawn，and Kruskal-Wallis test was used to compare the differences in colony growth between groups.

Results

Colony morphology of Mycobacterium tuberculosis was observed. After 14 days of culture，except 6180 strains，the other 5 strains had colony growth in NS group，with 50 ～100 colonies. After 20 days of Mycobacterium tuberculosis culture，6 strains in HCl group grew. After 26 days of Mycobacterium tuberculosis culture，except PAS group of 6545 strain，the other 5 strains grew. After 31 days of Mycobacterium tuberculosis culture，the size，shape and distribution of the 6 strains in NS group and HCl group were basically the same，while the distribution of the 6 strains in PAS group was less. The growth curves of 6 strains of Mycobacterium tuberculosis treated with normal saline，PAS and HCl showed that the growth trend of PAS group was lower than that of NS group and HCl group. Compared with the difference of Mycobacterium tuberculosis colony growth under normal saline，PAS and HCl treatment，the colony count of Mycobacterium tuberculosis in NS group was higher than that in PAS group （P＜0.05），and there was no statistical significance in the growth of Mycobacterium tuberculosis between NS group and HCl group （P＞0.05）.

Conclusion

Plasma activated saline has inhibitory effect on Mycobacterium tuberculosis in vitro experiment，showing great potential in anti-tuberculosis treatment，and may play a synergistic role in combined treatment to improve the therapeutic effect.

Key words: Mycobacterium tuberculosis, Plasma-activated physiological saline, Bacteriostatic action

Zhuo Wang, Shaoyi Qu, Yuanwu Zou, Qianhong Wu, Jing Li, Biao Wang, Zihan Wei, Xiaolin Wang. Inhibitory effect of plasma-activated physiological saline on Mycobacterium tuberculosis in vitro[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2025, 18(02): 279-283.

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Figures/Tables 4

References 25

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菌株编号	组　别	14 d	20 d	26 d	31 d
6180	NS	-	+	++	++
	PAS	-	-	+	+
	HCl	-	+	++	++
6943	NS	+	++	++	++
	PAS	-	+	++	++
	HCl	-	+	++	++
6545	NS	+	+	++	++
	PAS	-	-	-	-
	HCl	-	+	++	++
7154	NS	+	+	+++	+++
	PAS	-	-	+	+
	HCl	-	+	++	++
7158	NS	+	+	++	++
	PAS	-	+	+	+
	HCl	-	+	++	++
H37RV	NS	+	+++	+++	+++
	PAS	-	+	+	+
	HCl	+	+	++	++

菌株编号	组　别	14 d	20 d	26 d	31 d
6180	NS	-	+	++	++
	PAS	-	-	+	+
	HCl	-	+	++	++
6943	NS	+	++	++	++
	PAS	-	+	++	++
	HCl	-	+	++	++
6545	NS	+	+	++	++
	PAS	-	-	-	-
	HCl	-	+	++	++
7154	NS	+	+	+++	+++
	PAS	-	-	+	+
	HCl	-	+	++	++
7158	NS	+	+	++	++
	PAS	-	+	+	+
	HCl	-	+	++	++
H37RV	NS	+	+++	+++	+++
	PAS	-	+	+	+
	HCl	+	+	++	++

生长时间（d）	处理条件	P值
14	NS-PAS	0.000
	NS-HCl	0.000
	PAS-HCl	0.324
20	NS-PAS	0.000
	NS-HCl	0.333
	PAS-HCl	0.001
26	NS-PAS	0.000
	NS-HCl	0.231
	PAS-HCl	0.003
31	NS-PAS	0.007
	NS-HCl	0.016
	PAS-HCl	0.518

生长时间（d）	处理条件	P值
14	NS-PAS	0.000
	NS-HCl	0.000
	PAS-HCl	0.324
20	NS-PAS	0.000
	NS-HCl	0.333
	PAS-HCl	0.001
26	NS-PAS	0.000
	NS-HCl	0.231
	PAS-HCl	0.003
31	NS-PAS	0.007
	NS-HCl	0.016
	PAS-HCl	0.518

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