SDF-1α/CXCR4 signaling improves the therapeutic effect of bone marrow mesenchymal stem cells on seawater drowning induced acute lung injury

doi:10.3877/cma.j.issn.1674-6902.2019.01.005

Abstract

Abstract:

Objective

To investigate the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs) on seawater drowning induced acute lung injury (SWD-ALI) and the role of SDF-1α/CXCR4 signaling.

Methods

BMMSCs were isolated and cultured and a rat model of SWD-ALI was established. Then, the BMMSCs migration was tested by transwell, the SDF-1α/CXCR4 signaling was regulated by AMD3100 pretreatment and lung delivery of SDF-1α, the expressions of SDF-1α and CXCR4 in lung were detected by real-time PCR and western blot, and the HE staining and wet/dry ratio were used to determine the lung injury.

Results

BMMSCs alleviates the alveoli exudation, edema and inflammatory cell infiltration induced by seawater inhalation, which was abolished by the CXCR4 blocker. SDF-1α promoted the migration of BMMSCs in vitro, and facilitated the repairing action of BMMSCs to the SWD-ALI in vivo.

Conclusion

BMMSCs could effectively therapy the SWD-ALI, and SDF-1α/CXCR4 signaling play important roles in promoting the BMMSCs migration to the injured lung and subsequently repairing process.

Key words: Seawater, Lung injury, Bone marrow mesenchymal stem cells, SDF-1α, CXCR4

Sidi Song, Xi Lu, Faguang Jin, Yuan Liang, Gaole Yu. SDF-1α/CXCR4 signaling improves the therapeutic effect of bone marrow mesenchymal stem cells on seawater drowning induced acute lung injury[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2019, 12(01): 28-33.

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Figures/Tables 6

References 20

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名　称	序　列
SDF-1α F	TCTGCATCAGTGACGGTAAG
SDF-1α R	TAAGGCTTTGTCCAGGTA
CXCR4 F	TCTTCTTGACTGGCATAGTGG
CXCR4 R	GCTGTAAAGGTTGACGGTGTA
β-actin F	GGAGATTACTGCCCTGGCTCCTAGC
β-actin R	GGCCGGACTCATCGTACTCCTGCTT

名　称	序　列
SDF-1α F	TCTGCATCAGTGACGGTAAG
SDF-1α R	TAAGGCTTTGTCCAGGTA
CXCR4 F	TCTTCTTGACTGGCATAGTGG
CXCR4 R	GCTGTAAAGGTTGACGGTGTA
β-actin F	GGAGATTACTGCCCTGGCTCCTAGC
β-actin R	GGCCGGACTCATCGTACTCCTGCTT

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