Expression of Nix after mechanical stretch-induced acute lung injury and its correlation with injury severity

doi:10.3877/cma.j.issn.1674-6902.2020.03.010

Abstract

Abstract:

Objective

To investigate the correlation between the expression and injury severity of Nix after acute lung injury induced by mechanical stretch.

Methods

A small biological impact instrument accurately controlled the establishment of rat chest impact injury models with different injury severities: 10 rats with minor injury (the minor injury group), 10 with moderate injury (the moderate injury group), and 10 with serious injury (the serious injury group). And 10 rats received no treatment as the control group. Then lung tissues of all the groups were taken with no injury 0.5, 2, 12, 24, 48, and 72 h after the precise impact, respectively, and HE staining was employed to observe the pathological changes. The AT-Ⅱ cell culture strains with different degrees of injury were taken and RT-PCR was used to detect the expression of Nix in the alveolar cells at each time point. The expression levels of proteins were detected by Western blotting method.

Results

The lung tissues of the injury groups showed different degrees of hemorrhage, edema and massive inflammatory cell infiltration. Compared with the control group, after the impact for 0.5, 2, 12, 24, 48, and 72 h, the expression of Nix mRNA in the lung tissues of each injury group was significantly increased, and Nix mRNA expression began to increase 0.5 h after injury. The expression of Nix mRNA peaked at 48 h after impact, and decreased significantly at 72 h after impact (P<0.05), which was close to that of 0.5 h after injury. And the expression levels of Nix mRNA increased correspondingly with the severity of impact injury (P<0.05). Compared with the control group, the expression of Nix proteins in the lung tissues of each injury group also showed the same change curve with the expression level of Nix mRNA (P<0.05), and also increased with the severity of impact injury (P<0.05).

Conclusion

In the rats with acute lung injury induced by mechanical stretch, the expression of Nix gene in the lung tissues was positively correlated with the severity of lung injury (P<0.05). This provides a theoretical basis for our study on lung tissue-related diseases through Nix as a target.

Key words: Acute lung injury, Nix, Mechanical stretch, Chest impact injury

Zhiyuan Lai, Zhenyu Liu, Xiujiang Li, Qiangping Zhou. Expression of Nix after mechanical stretch-induced acute lung injury and its correlation with injury severity[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2020, 13(03): 340-344.

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References 34

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组　别	0.5 h	2 h	12 h	24 h	48 h	72 h
对照组	0.051±0.0046	0.051±0.0054	0.051±0.0073	0.051±0.0062	0.051±0.0046	0.051±0.0082
轻伤	0.368±0.072	0.487±0.083	0.652±0.098	0.691±0.096	0.952±0.082	0.342±0.043
重伤	0.569±0.071	0.673±0.064	0.752±0.091	0.891±0.099	1.491±0.072	0.300±0.051
严重伤	0.638±0.072	0.885±0.072	0.922±0.063	0.998±0.098	2.658±0.092	0.640±0.052

组　别	0.5 h	2 h	12 h	24 h	48 h	72 h
对照组	0.051±0.0046	0.051±0.0054	0.051±0.0073	0.051±0.0062	0.051±0.0046	0.051±0.0082
轻伤	0.368±0.072	0.487±0.083	0.652±0.098	0.691±0.096	0.952±0.082	0.342±0.043
重伤	0.569±0.071	0.673±0.064	0.752±0.091	0.891±0.099	1.491±0.072	0.300±0.051
严重伤	0.638±0.072	0.885±0.072	0.922±0.063	0.998±0.098	2.658±0.092	0.640±0.052

组　别	0.5 h	2 h	12 h	24 h	48 h	72 h
对照组	0.051±0.0036	0.051±0.0046	0.051±0.0146	0.051±0.0056	0.051±0.0066	0.051±0.0046
轻伤	0.361±0.072	0.487±0.083	0.652±0.091	0.691±0.093	1.111±0.082	0.342±0.043
重伤	0.360±0.072	0.492±0.073	0.699±0.094	0.721±0.096	1.562±0.062	0.396±0.049
严重伤	0.378±0.072	0.527±0.094	0.752±0.088	0.891±0.067	1.990±0.033	0.540±0.072

组　别	0.5 h	2 h	12 h	24 h	48 h	72 h
对照组	0.051±0.0036	0.051±0.0046	0.051±0.0146	0.051±0.0056	0.051±0.0066	0.051±0.0046
轻伤	0.361±0.072	0.487±0.083	0.652±0.091	0.691±0.093	1.111±0.082	0.342±0.043
重伤	0.360±0.072	0.492±0.073	0.699±0.094	0.721±0.096	1.562±0.062	0.396±0.049
严重伤	0.378±0.072	0.527±0.094	0.752±0.088	0.891±0.067	1.990±0.033	0.540±0.072

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