miR-200b-3p suppressed the edema in seawater aspiration-induced ALI via inhibition the expression of HIF-1α

doi:10.3877/cma.j.issn.1674-6902.2024.05.005

Abstract

Abstract:

Objective

To analyze the role and mechanism of miR-200b-3p in seawater inhalation acute lung injury （ALI）.

Methods

Thirty-two healthy SD rats were selected and divided into blank control group， seawater stimulation group， miR-200b-3p antagomir pretreatment group and antag NC pretreatment group， with 8 rats in each group. The rat pulmonary microvascular endothelial cells （RPMVECs） model of seawater inhalation lung injury and rat pulmonary microvascular endothelial cells were constructed. Wet-dry ratio（W/D） of lung tissue was observed， and microvascular permeability of lung was detected by Evans blue method. Western blot analysis of the expression of -hypoxia inducible factor-1（HIF-1α） and vascular endothelial growth factor （VEGF） were detected. Dual luciferase reporter gene assay was used to verify the relationship between HIF-1α and miR-200b-3p.

Results

After seawater treatment for 4 h， the wet-dry ratio（W/D） and permeability of lung tissue were significantly increased， and the expression of miR-200b-3p， HIF-1α and VEGF were significantly increased in lung tissue and cell models. However， inhibition of the expression of miR-200b-3p significantly alleviated pulmonary edema， the expression of HIF-1α and VEGF were decreased in lung tissues and cell models. Dual luciferase reporter gene verified that miR-200b-3p directly targeted HIF-1α. Compared with blank control group， the expressions of HIF-1α and VEGF in lung tissue and HIF-1α and VEGF in RPMVECs cells were increased in seawater stimulated group.

Conclusion

Increased expression of miR-200b-3p plays a key role in the development of pulmonary edema induced by seawater aspiration injury，which is mediated by the HIF-1α/VEGF pathway.

Key words: Acute lung injury, Seawater, Hypoxia inducible factor-1/vascular endothelial growth factor pathway, miR-200b-3p

Minlong Zhang, Cuiping Yang, Bo Wang, Yunjie Cui, Faguang Jin. miR-200b-3p suppressed the edema in seawater aspiration-induced ALI via inhibition the expression of HIF-1α[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2024, 17(05): 696-700.

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URL: https://zhfbjbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-6902.2024.05.005

https://zhfbjbzz.cma-cmc.com.cn/EN/Y2024/V17/I05/696

Figures/Tables 5

References 24

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