特发性肺纤维化患者诱导痰中SDF-1的表达与高分辨CT表现的相关性研究

doi:10.3877/cma.j.issn.1674-6902.2017.03.011

中华肺部疾病杂志(电子版) ›› 2017, Vol. 10 ›› Issue (03) : 296 -299. doi: 10.3877/cma.j.issn.1674-6902.2017.03.011

所属专题：文献；

论著

特发性肺纤维化患者诱导痰中SDF-1的表达与高分辨CT表现的相关性研究

吴东¹, 袁亚连¹, 陈敏¹, 李文¹, 吕权超¹, 黎东明¹^,^†(

)

^1. 524001　湛江，广东医科大学附属医院呼吸疾病研究所·呼吸内科

收稿日期:2017-03-20 出版日期:2017-06-20
通信作者: 黎东明
基金资助:
广东省高校优秀青年创新人才培育基金项目(LYM09086)

Correlation between the high-resolution computed tomography parameters and levels of stromal cell-derived factor 1 in induced sputum of idiopathic pulmonary fibrosis patients

Dong Wu¹, Yalian Yuan¹, Min Chen¹, Wen LI¹, Quanchao Lv¹, Dongming Li¹^,^†()

^1. Institute of Respiratory Diseases, Department of Respiratory, The Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China

Received:2017-03-20 Published:2017-06-20
Corresponding author: Dongming Li
About author:
Corresponding author: Li Dongming, Email: 3064264@qq.com

全文 ( ) 下载PDF ( ) 导出引用

目的

探讨特发性肺纤维化(IPF)患者血清和诱导痰中基质细胞衍生因子-1(SDF-1)的表达水平与肺功能及胸部高分辨CT表现的相关性。

方法

选取IPF患者32例及健康体检者30例，收集血清和诱导痰标本并检测SDF-1表达水平，IPF患者行肺功能和胸部高分辨CT检查，并对结果作相关分析。

结果

IPF患者的诱导痰中SDF-1的表达水平为(705.21±11.71)ng/ml，高于健康体检组的(252.82±10.62)ng/ml，P<0.05差异有统计学意义。血清中SDF-1的表达水平(5.29±0.13)ng/ml高于健康体检组的(4.93±0.14)ng/ml，但P>0.05，没有统计学意义。IPF患者肺功能均有限制性通气功能障碍和弥散功能障碍；影像学表现为程度不一的磨玻璃影、网格影和蜂窝肺，HRCT评分与诱导痰中的SDF-1表达水平呈正相关(r＝0.287，P<0.05)。

结论

SDF-1在IPF患者诱导痰中表达明显升高，并与患者病情严重程度(胸部高分辨CT表现)呈正相关，对IPF诊断和治疗具有潜在的参考价值。

关键词: 特发性肺纤维化, 基质细胞衍生因子-1, 肺功能, 高分辨CT

Objective

To evaluate concentrates of stromal cell-derived factor in serum and induced sputum supernatants from idiopathic pulmonary fibrosis(IPF) patients, investigate the relationship between the stromal cell-derived factor-1 (SDF-1) level and the high-resolution computed tomography parameters in patients and explore the role of SDF-1 in the diagnosis and treatment of IPF.

Methods

Serum and induced sputum samples were collected from 30 healthy controls and 32 patients with IPF. The concentrations of SDF-1 in serum and induced sputum were measured by ELISA. Lung function and chest high-resolution CT were executed in patients with IPF. Analysis of correlation was performed on these results.

Results

The concentration of SDF-1 in induced sputum was significantly increased in IPF group(705.21±11.71)ng/ml compared with healthy group(252.82±10.62)ng/ml(P<0.05). The level of SDF-1 in serum was also elevated in IPF patients(5.29±0.13)ng/ml compared with healthy group(4.93±0.14)ng/ml, but there was no statistically significant difference (P>0.05). All patients with IPF have restrictive ventilation dysfunction and diffusion dysfunction. There was a positive relationship between induced sputum SDF-1 level and HRCT score(r=0.287, P<0.01).

Conclusions

The expression of SDF-1 in induced sputum was significantly increased, and associated positively with severe degree (chest high-resolution CT manifestations) of patients with IPF. SDF-1 may play an important role in the pathogenesis of IPF, therefor serve as a potent testing index of the diagnosis and treatment of IPF.

Key words: Idiopathic pulmonary fibrosis, Stromal cell-derived factor-1, Lung function, High-resolution computed tomography

图1 SDF-1在两组血清和诱导痰中的表达水平

表1 两组的肺功能参数(±s)

参　数	FVC%预计值	FEV₁%预计值	DLCO%预计值
健康体检者	108.58±11.03	98.67±13.56	101.23＋9.85
IPF患者	63.3±15.24	71.52±17.75	57.52＋20.15

表1 两组的肺功能参数(±s)

参　数	FVC%预计值	FEV₁%预计值	DLCO%预计值
健康体检者	108.58±11.03	98.67±13.56	101.23＋9.85
IPF患者	63.3±15.24	71.52±17.75	57.52＋20.15

图2 CT评分与诱导痰中SDF-1含量的相关性

图3 代表性病例：男性，59岁，因"进行性呼吸困难2年，加重10天"入院，经纤维支气管镜肺活检结果符合特发性肺纤维化改变；HRCT结果显示双肺弥漫性蜂窝样改变伴双下肺牵拉性支气管扩张；肺功能检查示中度限制性通气功能障碍和重度弥散功能障碍

1	崔社怀. 特发性肺间质纤维化的回顾和展望[J/CD]. 中华肺部疾病杂志(电子版), 2010, 3(1): 4-5.
2	Nakagawa H, Nagatani Y, Takahashi M, et al. Quantitative CT analysis of honeycombing area in idiopathic pulmonary fibrosis: Correlations with pulmonary function tests[J]. Eur J Radiol, 2016, 85(1): 125-130.
3	Nishiyama O, Tohda Y. Obstructive lung function in idiopathic pulmonary fibrosis[J]. Chron Respir Dis, 2016, 13(2): 206.
4	李文，吴东，梁标. 基质细胞衍生因子-1与肺纤维化关系的初步探讨[J]. 中国现代医学杂志，2008, 18(11): 1545-1547.
5	Raghu G, Collard HR, Egan JJ, et al. An official ATS/ERS/JRS/ALAT statement: idiopathic pulmonary fibrosis: evidence-based guidelines for diagnosis and management[J]. Am J Respir Crit Care Med, 2011, 183(6): 788-824.
6	张红军，顾兴，刘伟，等. 特发性肺间质纤维化患者肿瘤标志物的特点分析[J/CD]. 中华肺部疾病杂志(电子版), 2016, 9(6): 621-624.
7	Vignola AM, Rennar SI, Hargreave FE, et al. Standardised methodology of sputum induction and processing. Future directions[J]．Eur Respir J Suppl, 2002, 37: 51s-55s.
8	Behr J, Demedts M, Buhl R, et al. Lung function in idiopathic pulmonary fibrosis-extended analyses of the IFIGENIA trial[J]. Respir Res, 2009, 10: 101.
9	吴友茹，陈明勇，朱静. 肺纤维化患者血清及诱导痰肺表面活性蛋白水平测定及意义[J]. 检验医学与临床，2015, 12(3): 350-351.
10	Juarez J, Bendall L, Bradstock K. Chemokines and their receptors as therapeutic targets: the role of the SDF-1/CXCR4 axis[J]. Curr Pharm Des, 2004, 10(11): 1245-1259.
11	王坤，赵沣凯，李彦林，等. T140体内靶向阻断SDF-1/CXCR4信号通路对SDF-1及MMPs的影响[J]. 实用医学杂志，2015, 31(19): 3133-3136.
12	Xu J, Mora A, Shim H, et al. Role of the SDF-1/CXCR4 axis in the pathogenesis of lung injury and fibrosis[J]. Am J Respir Cell Mol Biol, 2007, 37(3): 291-299.
13	李惠萍. 特发性肺纤维化的诊断和治疗[J]. 中华结核和呼吸杂志，2014, 37(9): 64.
14	郭晓娟，包娜，张镭，等. 特发性间质性肺炎高分辨率CT征象特点的量化比较分析[J]. 中华医学杂志，2011, 91(1): 11-15.
15	李文，吴东，罗伟仁. 姜黄素对哮喘大鼠肺内胶原沉积、结缔组织生长因子表达及气道重构的影响[J]. 实用医学杂志，2008, 24(15): 2562-2564.

[1]	杨茂宪, 沈鹏, 王倩倩, 吴旺, 沈永帅, 蒋禛, 徐龙生, 朱建刚, 刘倍倍. 吡啶甲酸镁联合地塞米松对急性呼吸窘迫综合征大鼠的治疗作用研究[J]. 中华危重症医学杂志(电子版), 2024, 17(03): 196-203.
[2]	吴越廷, 周林雨涵, 胡钦, 许华燕, 黄敏, 陈晓勇, 张萌, 李中会, 茹凉, 王秋, 蔡晓唐. 皮质类固醇治疗非卧床杜氏肌营养不良症患儿肺功能与运动功能的纵向研究[J]. 中华妇幼临床医学杂志(电子版), 2024, 20(03): 292-301.
[3]	阙宏亮, 邓君鹏, 李权, 曾腾跃, 沈华, 谢建军. 俯卧位经后腹腔肾上腺腹腔镜手术的研究进展[J]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(02): 188-192.
[4]	张璇, 高杨, 房雅君, 姚艳玲. 保护性机械通气在肺癌胸腔镜肺段切除术中的临床应用[J]. 中华肺部疾病杂志(电子版), 2024, 17(04): 563-567.
[5]	陈冬丽, 邓迎丽, 毕婧. α-干扰素治疗急性呼吸道病毒感染对Th1/Th2平衡及肺功能的影响[J]. 中华肺部疾病杂志(电子版), 2024, 17(04): 590-594.
[6]	王微, 丁霖, 陈茜, 呼延欣, 闫蓓. 综合治疗对慢性阻塞性肺疾病的疗效及转归影响分析[J]. 中华肺部疾病杂志(电子版), 2024, 17(04): 621-624.
[7]	袁广琴, 朱珠, 林云霞. 胸腺肽联合无创正压通气救治AECOPD并发Ⅱ型呼吸衰竭患者的临床分析[J]. 中华肺部疾病杂志(电子版), 2024, 17(03): 438-441.
[8]	周璇, 谢莉, 邹娟. 尼达尼布对特发性肺纤维化肺功能、肺纤维化程度及PDGF、PGE2、TGF-β1的影响[J]. 中华肺部疾病杂志(电子版), 2024, 17(03): 368-372.
[9]	罗孝平, 封敏, 黄川, 唐茜, 蒋艳, 胡莉丽. 渐进式抗阻训练干预在非小细胞肺癌中的临床应用[J]. 中华肺部疾病杂志(电子版), 2024, 17(03): 472-474.
[10]	邱凌霄, 王创业, 卿斌, 刘锦程, 张鑫烨, 武文娟, 邢德冰, 郭亮, 徐智, 王斌. 基于转录组学筛选特发性肺纤维化的枢纽基因和信号通路[J]. 中华肺部疾病杂志(电子版), 2024, 17(02): 212-217.
[11]	吴沛玲, 娄月妍, 张洪艳, 陈东方, 刘雪青, 赵丽芳, 薛姗, 蒋捍东. 线粒体相关基因在特发性肺纤维化中的分析[J]. 中华肺部疾病杂志(电子版), 2024, 17(02): 178-184.
[12]	拉周措毛, 山春玲, 李国蓉, 华毛. 青海西宁地区IPF-LC的病理类型及临床特征分析[J]. 中华肺部疾病杂志(电子版), 2024, 17(01): 25-29.
[13]	陈先志, 许磊, 冯其柱, 王琦. 布地奈德联合复方异丙托溴铵雾化吸入在老年患者腹腔镜围手术期中的应用[J]. 中华肝脏外科手术学电子杂志, 2024, 13(04): 531-536.
[14]	闫维, 张二明, 张克, 安欣华, 向平超. 北京市石景山区40岁及以上居民早期慢性阻塞性肺疾病异质性及影响因素分析[J]. 中华临床医师杂志(电子版), 2024, 18(06): 533-540.
[15]	张克, 张二明, 闫维, 马石头, 安欣华, 包曹歆, 向平超. 北京石景山区40岁以上PRISm和COPD患者的肺功能水平与吸烟情况相关性分析[J]. 中华临床医师杂志(电子版), 2024, 18(02): 122-127.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

Correlation between the high-resolution computed tomography parameters and levels of stromal cell-derived factor 1 in induced sputum of idiopathic pulmonary fibrosis patients

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

Correlation between the high-resolution computed tomography parameters and levels of stromal cell-derived factor 1 in induced sputum of idiopathic pulmonary fibrosis patients