Protective effect of ANGPTL4 on human lung microvascular endothelial cell injury

doi:10.3877/cma.j.issn.1674-6902.2017.02.016

Abstract

Abstract:

Objective

To investigate the protective effect of ANGPTL4 to human lung microvascular endothelial cell injury.

Method

Human lung microvascular endothelial cells cultured in vitro were randomly divided into four groups: normal control group (group C), LPS induced group (group L), ANGPTL4 expression (group A) and ANGPTL4+ LPS induced group (group A+ L). CCK8 method was used to observe cell proliferation; fluorescence quantitative PCR and Western blot were applied to test ANGPTL4 expression level, Inflammatory factor TNF-α, IL-1β and MCP-1in cell culture fluid were also detected by ELISA method.

Results

Compared with group C, the expression of ANGPTL4 in group L and group A was significantly higher(P<0.01); Compared with group L, The expression of ANGPTL4 in A+ L group was significantly higher (P<0.01), LPS stimulation can inhibit cell proliferation, and after pcDNA3.1- ANGPTL4 recombinant plasmid transfection, this inhibition was weakened; high ANGPTL4 expression can also inhibit the generation of inflammatory factor TNF-α, IL-1β and MCP-1.

Conclusions

ANGPTL4 can inhibit LPS induced HPMVECs cell proliferation decreased, and reduce the generation of Inflammatory factor TNF-α, IL-1β and MCP-1, which may be one of the mechanism of the protective effect of ANGPTL4 to human lung microvascular endothelial cells.

Key words: ANGPTL4, Acute lung injury, Human lung microvascular endothelial cell

Yongmei Zhao, Rui Wang, Xinyu Li, Zhigao He, Junhao Gui, Donghui Zhang, Xiaoyan Wang, Feng Zhang, Yu Zhang, Peng Jiang. Protective effect of ANGPTL4 on human lung microvascular endothelial cell injury[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2017, 10(02): 192-195.

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URL: https://zhfbjbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-6902.2017.02.016

https://zhfbjbzz.cma-cmc.com.cn/EN/Y2017/V10/I02/192

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References 21

1	Fukuda Y. Acute lung injury/acute respiratory distress syndrome: progress in diagnosis and treatment. Topics: I. Pathogenesis and pathophysiology: 4. Pathophysiology and histopathology of ALI/ARDS[J]. Nihon Naika Gakkai Zasshi, 2011, 100(6): 1536-1540.
2	金发光. 急性肺损伤的诊治研究现状及进展[J/CD]. 中华肺部疾病杂志(电子版), 2013, 6(1): 1-3.
3	施卉，任成山. 急性肺损伤/急性呼吸窘迫综合征基础及临床研究进展[J/CD]. 中华肺部疾病杂志(电子版), 2013, 6(4): 350-355.
4	Pittet JF, Mackersie RC, Martin TR, et al. Biological markers of acute lung injury: prognostic and pathogenetic significance [J]. Am J Respir Crit Care Med, 1997, 155(4): 1187-1205.
5	Puthanveetil P, Wan A, Rodrigues B. Lipoprotein lipase and angiopoietin-like 4-cardiomyocyte secretory proteins that regulate metabolism during diabetic heart disease[J]. Crit Rev Clin Lab Sci, 2015, 52(3): 138-149.
6	Chi X, Shetty SK, Shows HW, et al. Angiopoietin-like 4 modifies the interactions between lipoprotein lipase and its endothelial cell transporter GPIHBP1[J]. J Biol Chem, 2015, 290(19): 11865-11877.
7	Zhu P, Goh YY, Chin HF, et al. Angiopoietin-like 4: a decade of research[J]. Biosci Rep, 2012, 32(3): 211-219.
8	李晓瑜，王浩，方芳，等. 异丙酚预处理对脂多糖(LPS)诱导的人肺微血管内皮细胞(HPMVCs)血管紧张素转化酶(ACE)的表达与活性的影响[J]. 复旦学报(医学版), 2013, 40(6): 668-672.
9	赵咏梅，王瑞，李新宇，等. ANGPTL4免疫脂质体对急性肺损伤小鼠的治疗作用[J/CD]. 中华肺部疾病杂志(电子版), 2016, 9(5): 507-511.
10	Stevens T. Functional and molecular heterogeneity of pulmonary endothelial cells[J]. Proc Am Thorac Soc, 2011, 8(6): 453-457.
11	He XY, Shi XY, Yuan HB, et al. Propofol attenuates hypoxia-induced apoptosis in alveolar epithelial type Ⅱ cells through down-regulating hypoxia-inducible factor-1α[J]. Injury, 2012, 43(3): 279-283.
12	Ozkan F, Senayli Y, Ozyurt H, et al. Antioxidant effects of propofol on tourniquet-induced ischemia-reperfusion injury: an experimental study[J]. J Surg Res, 2012, 176(2): 601-617.
13	Klopper JP, Berenz A, Hays WR, et al. In vivo and microarray analysis of rexinoid-responsive anaplastic thyroid carcinoma[J]. Clin Cancer Res, 2008, 14(2): 589-596.
14	Ge H, Cha JY, Gopal H, et al. Differential regulation and properties of angiopoietin-like proteins 3 and 4[J]. J Lipid Res, 2005, 46(7): 1484-1490.
15	Wang Y, Chen H, Li H, et al. Effect of angiopoietin-like protein 4 on rat pulmonary microvascular endothelial cells exposed to LPS[J]. Int J Mol Med, 2013, 32(3): 568-576.
16	Orfanos SE, Mavrommati I, Korovesi I, et al. Pulmonary endothelium in acute lung injury: from basic science to the critically ill[J]. Intensive Care Med, 2004, 30(9): 1702-1714.
17	Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respiratory distress syndrome[J]. J Pathol, 2004, 202(2): 145-156.
18	Tauseef M, Knezevic N, Chava KR, et al. TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation[J]. J Exp Med, 2012, 209(11): 1953-1968.
19	于晓丽，田军，吕向光. 单核细胞趋化因子-1的作用及与炎性病变的关系[J]. 国际耳鼻咽喉头颈外科杂志，2012, 36(4): 232-234.
20	Liu WL, Liu ZW, Li TS, et al. Hydrogen sulfide donor regulates alveolar epithelial cell apoptosis in rats with acute lung injury[J]. Chin Med J (Engl), 2013, 126(3): 494-499.
21	Shelton JL, Wang L, Cepinskas G, et al. Human neutrophil-pulmonary microvascular endothelial cell interactions in vitro: differential effects of nitric oxide vs. peroxynitrite[J]. Microvasc Res, 2008, 76(2): 80-88.

基因	引物	引物序列
ANGPTL4	ANGPTL4_F	5′-ACAACTCAAGGCTCAGAA-3′
?	ANGPTL4_R	5′-CTCATGGTCTAGGTGCTT-3′
ALB	ALB_F	5′-TGAAGGTCGGAGTCAACGGATTTG-3′
?	ALB_R	5′-ATGTGGGCCATGAGGTCCACCAC-3′
FOXP2	FOXP2_F	5′-TCCATCAAAGAAACATGACTAAATGC-3′
?	FOXP2_R	5′-GTCAACTCCCTGAGGATAGGAACAT-3′

基因	引物	引物序列
ANGPTL4	ANGPTL4_F	5′-ACAACTCAAGGCTCAGAA-3′
?	ANGPTL4_R	5′-CTCATGGTCTAGGTGCTT-3′
ALB	ALB_F	5′-TGAAGGTCGGAGTCAACGGATTTG-3′
?	ALB_R	5′-ATGTGGGCCATGAGGTCCACCAC-3′
FOXP2	FOXP2_F	5′-TCCATCAAAGAAACATGACTAAATGC-3′
?	FOXP2_R	5′-GTCAACTCCCTGAGGATAGGAACAT-3′

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