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中华肺部疾病杂志(电子版) ›› 2017, Vol. 10 ›› Issue (06) : 686 -689. doi: 10.3877/cma.j.issn.1674-6902.2017.06.011

所属专题: 文献

论著

血浆游离DNA在非小细胞肺癌诊断中的价值
杜洁1,(), 隋永杰1, 李红艳1, 王水利1   
  1. 1. 710068 西安,陕西省人民医院健康体检中心
  • 收稿日期:2017-07-16 出版日期:2017-12-20
  • 通信作者: 杜洁
  • 基金资助:
    陕西省科技厅2013年科技惠民专项(2013K14-02-09)

Value of plasma free DNA in the diagnosis of non-small cell lung cancer

Jie Du1,(), Yongjie Sui1, Hongyan Li1, Shuili Wang1   

  1. 1. Medical examination center, Shanxi Provincial People′s Hospital, Xi′an 710068, China
  • Received:2017-07-16 Published:2017-12-20
  • Corresponding author: Jie Du
  • About author:
    Corresponding author: Du Jie, Email:
引用本文:

杜洁, 隋永杰, 李红艳, 王水利. 血浆游离DNA在非小细胞肺癌诊断中的价值[J]. 中华肺部疾病杂志(电子版), 2017, 10(06): 686-689.

Jie Du, Yongjie Sui, Hongyan Li, Shuili Wang. Value of plasma free DNA in the diagnosis of non-small cell lung cancer[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2017, 10(06): 686-689.

目的

探讨血浆游离DNA水平在非小细胞肺癌(non-small cell lung cancer, NSCLC)中的诊断价值。

方法

选取陕西省人民医院健康中心120例健康对照者,并提取收治呼吸内科的116例非小细胞肺癌患者的,采用荧光定量PCR方法检测血浆游离DNA水平。

结果

非小细胞肺癌组患者的血浆游离DNA水平为200.70±88.54 ng/ml,健康对照组的血浆游离DNA水平为18.65±6.30 ng/ml,组间比较差异有统计学意义(P<0.01);Ⅰ/Ⅱ期非小细胞肺癌患者的血浆游离DNA水平为172.75±72.87 ng/ml,Ⅲ/Ⅳ期非小细胞肺癌血浆游离DNA水平为221.88±93.86 ng/ml,组间比较差异有统计学意义(P<0.05)。血浆游离DNA水平与患者的年龄、性别、吸烟状况及组织学类型无明显相关性(P>0.05)。

结论

血浆游离DNA是一种新发现的肿瘤标志物,可作为非小细胞肺癌早期诊断和临床分期的有效工具。

Objective

To evaluate the value of plasma free DNA in the diagnosis of non-small cell lung cancer (NSCLC).

Methods

Plasma was collected from 116 patients with non-small cell lung cancer admitted to respiratory department, and 120 healthy subjects from Medical examination center, Shanxi Provincial People′s Hospital. Plasma free DNA was extracted and detected by fluorescence quantitative polymerase chain reaction, PCR.

Results

The plasma free DNA level of healthy people was (18.65±6.30) ng/ml, and the plasma free DNA level in patients with non-small cell lung cancer was (200.70±88.54) ng/ml, the difference was statistically significant(P<0.01). The plasma free DNA content of stage Ⅰ/Ⅱ non-small cell lung cancer was (172.75±72.87) ng/ml, and the plasma circulating DNA content in stage Ⅲ/Ⅳ non-small cell lung cancer was (221.88±93.86) ng/ml, (P<0.05). There was no significant correlation between sex, age, smoking status and histological type (P>0.05).

Conclusion

Plasma circulating DNA is a potentially valuable biomarker that may be an effective tool for early diagnosis and clinical staging of non-small cell lung cancer.

图1 标准曲线图
表1 NSCLC患者血浆游离DNA水平与临床参数及组织学类型的关系(ng/ml)
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