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中华肺部疾病杂志(电子版) ›› 2017, Vol. 10 ›› Issue (06) : 709 -712. doi: 10.3877/cma.j.issn.1674-6902.2017.06.016

所属专题: 文献

论著

支气管肺癌组织中包含氧化还原酶的WW域还原蛋白、同源性磷酸酶-张力蛋白的表达及临床意义
张竞1, 董周寰2, 赵明1, 薛志强1, 王钰琦1, 田晓东1, 王云喜1,()   
  1. 1. 100853 北京,解放军总医院胸外科
    2. 100853 北京,解放军总医院病理科
  • 收稿日期:2017-10-29 出版日期:2017-12-20
  • 通信作者: 王云喜

Expression and clinical significance of WWOX and PTEN protein in bronchogenic carcinoma

Jin Zhang1, Zhouhuan Dong2, Ming Zhao1, Zhiqiang Xue1, Yuqi Wang1, Xiaodong Tian1, Yunxi Wang1,()   

  1. 1. Department of Thoracic Surgery, General Hospital of the PLA, Beijing 100853 China
    2. Department of pathology, General Hospital of the PLA, Beijing 100853 China
  • Received:2017-10-29 Published:2017-12-20
  • Corresponding author: Yunxi Wang
  • About author:
    Corresponding author: Wang Yunxi, Email:
引用本文:

张竞, 董周寰, 赵明, 薛志强, 王钰琦, 田晓东, 王云喜. 支气管肺癌组织中包含氧化还原酶的WW域还原蛋白、同源性磷酸酶-张力蛋白的表达及临床意义[J]. 中华肺部疾病杂志(电子版), 2017, 10(06): 709-712.

Jin Zhang, Zhouhuan Dong, Ming Zhao, Zhiqiang Xue, Yuqi Wang, Xiaodong Tian, Yunxi Wang. Expression and clinical significance of WWOX and PTEN protein in bronchogenic carcinoma[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2017, 10(06): 709-712.

目的

探讨包含氧化还原酶的WW域抗原(WWOX)蛋白与同源性磷酸酶-张力蛋白(PTEN)在支气管肺癌组织中的表达及临床意义。

方法

应用免疫组化法检测WWOX与PTEN蛋白在87例支气管肺癌组织标本与23例正常肺组织标本中的表达,分析WWOX与PTEN蛋白表达与支气管肺癌患者临床病理特征之间的关系,并探讨两者在支气管肺癌组织中的表达相关性。

结果

WWOX与PTEN蛋白在支气管肺癌组织中的的阳性表达均显著低于正常组织中的表达(χ2=14.810,P<0.001;χ2=17.363,P<0.001)。WWOX蛋白表达与患者的性别、癌组织分化程度、临床分期、是否发生淋巴结转移均有关(P<0.05),但与年龄、肿瘤大小无关(P>0.05);而PTEN蛋白则只与是否发生淋巴结转移有关(P<0.05),与其他临床病理参数均无关(P>0.05)。WWOX与PTEN蛋白的表达之间不存在关联性(χ2=2.098,P=0.147)。

结论

WWOX与PTEN蛋白可能都参与了支气管肺癌的发生与恶性发展,但两者是通过不同的途径发生作用。

Objective

To investigate the expression of WW domain containing oxidoreductase (WWOX) and phosphatase and tension homology deleted on chromosome ten(PTEN) in bronchogenic carcinoma and their clinical significance, as well as their relationship.

Methods

It was detected that the expression of WWOX and PTEN in lung tissues from 87 patients with bronchogenic carcinoma and 23 healthy lung tissues by immunohistochemical analysis.

Results

The positive expression rates of both WWOX and PTEN were lower in cancer tissues than in normal lung tissues (χ2=14.810, P=0.000; χ2=17.363, P<0.001). Also, the expression of WWOX was related with gender, differentiation, clinical stages, lymph node metastasis (P<0.05), not related with age and the tumor size (P>0.05); the expression of PTEN was only related with lymph node metastasis (P<0.05), but not related with other clinical significance (χ2=2.098, P<0.001). There was no significant relationship between the expression of WWOX and PTEN.

Conclusions

Both WWOX and PTEN proteins may be able to influence the occurrence and development of bronchogenic carcinoma, but it is more likely that they function through different pathways.

表1 WWOX、PTEN蛋白在肺癌与正常肺组织中的表达
表2 WWOX、PTEN蛋白表达与支气管肺癌临床病理特征的关系(n=87)
表3 WWOX与PTEN蛋白表达之间的关系
1
钱桂生. 肺癌不同病理类型发病率的变化情况及其原因[J/CD]. 中华肺部疾病杂志(电子版), 2011, 4(1):1-6.
2
Strassen U, Hofauer B, Matsuba Y, et al. Bronchogenic cancer: It still exists[J]. Laryngoscope, 2016, 126(3):638-642.
3
Bednarek AK, Keck-Waggoner CL, Daniel RL, et al. WWOX, the FRA16D gene, behaves as a suppressor of tumor growth[J]. Cancer Res, 2001, 61(22):8068-8073.
4
Mahajan NP, Whang YE, Mohler JL, et al. Activated tyrosine kinase Ack1 promotes prostate tumorigenesis: role of Ack1 in polyubiquitination of tumor suppressor Wwox[J]. Cancer Res, 2005, 65(22):10514-10523.
5
Chang NS, Pratt N, Heath J, et al. Hyaluronidase induction of a WW domain-containing oxidoreductase that enhances tumor necrosis factor cytotoxicity[J]. J Biol Chem, 2001, 276(5):3361-3370.
6
Kremer EJ, Pritchard M, Lynch M, et al. Mapping of DNA instability at the fragile X to a trinucleotide repeat sequence p(CCG)n[J]. Science, 1991, 252(5013):1711-1714.
7
Chen Z, Trotman LC, Shaffer D, et al. Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis[J]. Nature, 2005, 436(7051):725-730.
8
Heavey S, O′Byrne KJ, Gately K. Strategies for co-targeting the PI3K/AKT/mTOR pathway in NSCLC[J]. Cancer Treat Rev, 2014, 40(3):445-456.
9
Printz C. Lung cancer new leading cause of death for women in developed countries: Data reflects increased rates of smoking[J]. Cancer, 2015, 121(12):1911-1912.
10
Schrock MS, Huebner K. WWOX: a fragile tumor suppressor[J]. Exp Biol Med (Maywood), 2015, 240(3):296-304.
11
Puciennik E, Nowakowska M, Stpien A, et al. Alternating expression levels of WWOX tumor suppressor and cancer-related genes in patients with bladder cancer[J]. Oncol Lett, 2014, 8(5):2291-2297.
12
Huang YC, Hung WC, Chen WT, et al. Expression of WWOX and FHIT is downregulated by exposure to arsenite in human uroepithelial cells[J]. Toxicol Lett, 2013, 220(2):118-125.
13
Del MS, Husanie H, Iancu O, et al. WWOX and p53 dysregulation synergize to drive the development of osteosarcoma[J]. Cancer Res, 2016, 76(20):6107-6117.
14
Nowakowska M, Puciennik E, Wujcicka WI, et al. The correlation analysis of WWOX expression and cancer related genes in neuroblastoma-a real time RT-PCR study[J]. Acta Biochim Pol, 2014, 61(1):91-97.
15
Nowakowska M, Pospiech K, Lewandowska U, et al. Diverse effect of WWOX overexpression in HT29 and SW480 colon cancer cell lines[J]. Tumour Biol, 2014, 35(9):9291-9301.
16
Guler G, Uner A, Guler N, et al. The fragile genes FHIT and WWOX are inactivated coordinately in invasive breast carcinoma[J]. Cancer, 2004, 100(8):1605-1614.
17
Lin JT, Li HY, Chang NS, et al. WWOX suppresses prostate cancer cell progression through cyclin D1-mediated cell cycle arrest in the G1 phase[J]. Cell Cycle, 2015, 14(3):408-416.
18
Li G, Sun L, Mu Z, et al. Ectopic WWOX expression inhibits growth of 5 637 bladder cancer cell in vitro and in vivo[J]. Cell Biochem Biophys, 2015, 73(2):417-425.
19
Ekizoglu S, Bulut P, Karaman E, et al. Epigenetic and genetic alterations affect the WWOX gene in head and neck squamous cell carcinoma[J]. PLoS One, 2015, 10(1):e0115353.
20
Dahia PL. PTEN, a unique tumor suppressor gene[J]. Endocr Relat Cancer, 2000, 7(2):115-129.
21
Carnero A, Blanco-Aparicio C, Renner O, et al. The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications[J]. Curr Cancer Drug Targets, 2008, 8(3):187-198.
22
Sushma PS, Jamil K, Kumar PU, et al. PTEN and p16 genes as epigenetic biomarkers in oral squamous cell carcinoma (OSCC): a study on south Indian population[J]. Tumour Biol, 2016, 37(6):7625-7632.
23
Xiao WZ, Han DH, Wang F, et al. Relationships between PTEN gene mutations and prognosis in glioma: a meta-analysis[J]. Tumour Biol, 2014, 35(7):6687-6693.
24
Zhang X, Park JS, Park KH, et al. PTEN deficiency as a predictive biomarker of resistance to HER2-targeted therapy in advanced gastric cancer[J]. Oncology, 2015, 88(2):76-85.
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