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中华肺部疾病杂志(电子版) ›› 2022, Vol. 15 ›› Issue (04) : 468 -472. doi: 10.3877/cma.j.issn.1674-6902.2022.04.003

论著

COPD患者NLRP3炎症小体及TNF-α、HMGB1的表达及相互关系
刘欣1, 李艳敏2, 郑佳2, 赵商岐2, 唐晓慧2, 赵静3, 周文涛2, 周晓涛4,()   
  1. 1. 830011 乌鲁木齐,新疆医科大学基础医学院免疫学教研室;831100 昌吉,新疆昌吉职业技术学院
    2. 830011 乌鲁木齐,新疆医科大学基础医学院免疫学教研室
    3. 830011 乌鲁木齐,新疆医科大学第五附属医院呼吸科
  • 收稿日期:2022-04-11 出版日期:2022-08-25
  • 通信作者: 周晓涛
  • 基金资助:
    国家自然科学基金资助项目(81760656); 新疆维吾尔自治区自然科学基金资助项目(2018D01C299)

Expression of NLRP3 Inflammasome, TNF-α and HMGB1 in COPD Patients and Their Relationship

Xin Liu1, Yanmin Li2, Jia Zheng2, Shangqi Zhao2, Xiaohui Tang2, Jing Zhao3, Wentao Zhou2, Xiaotao Zhou4,()   

  1. 1. Department of Immunology, Basic Medical College of the Xinjiang Medical University, Urumqi 830011, China; Xinjiang Changji Vocational and Technical College, Changji 831100, China
    2. Department of Immunology, Basic Medical College of the Xinjiang Medical University, Urumqi 830011, China
    3. The Fifth Affilated Hospital of Xinjing Medical University, Urumqi 830011, China
    4. Xinjiang Changji Vocational and Technical College, Changji 831100, China
  • Received:2022-04-11 Published:2022-08-25
  • Corresponding author: Xiaotao Zhou
引用本文:

刘欣, 李艳敏, 郑佳, 赵商岐, 唐晓慧, 赵静, 周文涛, 周晓涛. COPD患者NLRP3炎症小体及TNF-α、HMGB1的表达及相互关系[J]. 中华肺部疾病杂志(电子版), 2022, 15(04): 468-472.

Xin Liu, Yanmin Li, Jia Zheng, Shangqi Zhao, Xiaohui Tang, Jing Zhao, Wentao Zhou, Xiaotao Zhou. Expression of NLRP3 Inflammasome, TNF-α and HMGB1 in COPD Patients and Their Relationship[J]. Chinese Journal of Lung Diseases(Electronic Edition), 2022, 15(04): 468-472.

目的

分析核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎症小体通路,早期炎症因子肿瘤坏死因子α(TNF-α),人高迁移率族蛋白B1(HMGB1)在慢性阻塞性肺疾病急性加重期(AECOPD)患者中的表达及相关性。

方法

选择2017年9月至2019年2月我院呼吸内科收治的AECOPD患者48例为观察组,健康者30例为对照组,采用实时荧光定量PCR技术检测NLRP3炎症小体组分NLRP3、ASC、Caspase1及其活化因子IL-1β、IL-18的mRNA表达,采用ELISA法检测血清中IL-1β、IL-18、TNF -α、HMGB1。

结果

观察组NLRP3mRNA、IL-18mRNA表达水平高于对照组(P<0.05);观察组血清中IL-1β、IL-18、TNF-α、HMGB1含量高于对照组(P<0.05);观察组NLRP3mRNA表达与IL-18mRNA表达呈负相关(P<0.05);血清中TNF-α、IL-1β、HMGB1之间表达呈正相关(P<0.05);病程>10年患者Caspase1 mRNA、血清IL-18和TNF-α的水平高于病程≤10年患者(P<0.05);Caspase1 mRNA与病程不相关(P>0.05),血清中IL-18及TNF-α与病程正相关(P<0.05),确诊年龄,移动度(右)与病程负相关(P<0.05);病程、移动度对Caspase1 mRNA不相关(P>0.05);移动度对血清IL-18和TNF-α不相关(P>0.05),病程与血清IL-18和TNF-α呈正相关(P<0.01)。

结论

NLRP3炎症小体相关指标、TNF-α及HMGB1参与AECOPD的炎症过程。血清中IL-1β、TNF-α和HMGB1可作为AECOPD的临床炎症诊断指标。病程越长,AECOPD体内Caspase1 mRNA、血清中TNF-α、IL-18含量越高,提示感染性炎症越明显。

Objective

To explore the expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome pathway, early inflammatory factor-tumor necrosis factor α(TNF-α), human high mobility group protein B1 (HMGB1) in the patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and their relationship.

Methods

A total of 48 AECOPD patients admitted to the respiratory department of our hospital from September 2017 to February 2019 were selected as the observation group, and 30 healthy patients as the control group. The mRNA level of the NLRP3 inflammasome components (NLRP3, ASC, CASP1 and its activating factors IL-1β and IL-18) was detected by Real-time fluorescent quantitative PCR technology, serum was prepared to detect the concentration of IL-1β, IL-18, TNF-α and HMGB1 by ELISA.

Results

The expression levels of NLRP3mRNA and IL-18 mrna in observation group were higher than those in control group (P<0.05). The contents of IL-1β, IL-18, TNF-α and HMGB1 in serum of observation group were higher than those of control group (P<0.05). NLRP3mRNA expression was negatively correlated with IL-18 mrna expression in observation group (P<0.05); The expressions of TNF-α, IL-1β and HMGB1 in serum were positively correlated (P<0.05). The levels of caspase1mRNA, serum IL-18 and TNF-α in the course of disease > 10 years group were higher than those in the course of disease ≤10 years group (P<0.05). Caspase1mRNA was not correlated with the course of disease (P>0.05), serum IL-18 and TNF-α were positively correlated with the course of disease (P<0.05), age of diagnosis, degree of mobility (right) were negatively correlated with the course of disease (P<0.05). There was no correlation between course of disease and degree of movement on caspase1 mRNA (P>0.05). The degree of mobility was not correlated with serum IL-18 and TNF-α (P>0.05), but the course of disease was positively correlated with serum IL-18 and TNF-α (P<0.01).

Conclusion

NLRP3 inflammasome related indexes, TNF-α and HMGB1 are involved in the inflammatory process of AECOPD. Serum IL-1β, TNF-α and HMGB1 can be considered as clinical inflammatory diagnostic indicators of AECOPD. The longer the course of disease, the higher the contents of caspase1mRNA, TNF-α and IL-18 in serum of AECOPD patients, indicating the more obvious infectious inflammation.

表1 两组NLRP3炎症小体相关指标、TNF-α及HMGB1表达水平(±s)
表2 疾病组不同病程指标差异性分析
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